A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
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骨髓细胞 (Trem) 家族成员 Trem-like 4 上表达的新触发受体可与死细胞结合，是小鼠巨噬细胞和树突状细胞亚群的 DNAX 激活蛋白 12 连锁标记。
DOI：
10.4049/jimmunol.182.3.1278
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发表时间：
2009-02-01
期刊：
影响因子：
--
通讯作者：
Steinman RM
中科院分区：
文献类型：
--
作者：
Hemmi H;Idoyaga J;Suda K;Suda N;Kennedy K;Noda M;Aderem A;Steinman RM
树突状细胞（Dendritic cells，DC）是专职的抗原呈递细胞，其可以控制针对自身和改变的自身（通常为外来）决定簇的免疫应答。DC可以分为几个亚群，包括CD 8 α+和CD 8 α− DC。这些子集具有特定的功能。例如，小鼠脾脏CD 8 α+而不是CD 8 α− DCs选择性地摄取垂死细胞并将细胞相关抗原交叉呈递给幼稚T细胞。在这项研究中，我们通过微阵列分析确定了在CD 8 α+ DCs中比在CD 8 α− DCs中表达更多的基因。当细胞外结构域与人IgG Fc结构域连接时，这些基因中只有一个可以与晚期凋亡或坏死细胞结合。该基因是髓系细胞表达的触发受体（Trem）家族的新成员Trem-like 4（Treml 4）。Treml 4 mRNA和蛋白质，后者检测到一种新的单克隆抗体，主要在脾脏中表达。Trem 14与其他Trem家族成员一样，可以与衔接分子DAP 12结合，但不能与DAP 10和FcRγ结合。与微阵列数据一致，我们证实Treml 4蛋白在CD 8 α+上比在CD 8 α− DCs上表达更多，并且我们还发现Treml 4在脾中的脾巨噬细胞上以高水平表达，特别是红髓和边缘嗜金属巨噬细胞。Toll样受体配体诱导DC成熟后，Treml 4表达无明显变化。因此，Treml 4是一个新的Trem家族分子，在CD 8 α+ DC和脾脏巨噬细胞亚群上大量表达，并能识别脾脏中不同类型的吞噬细胞死亡细胞。
Dendritic cells (DCs) are professional antigen presenting cells that can control immune responses against self and altered self, typically foreign, determinants. DCs can be divided into several subsets including CD8α+ and CD8α− DCs. These subsets possess specific functions. For example, mouse splenic CD8α+, but not CD8α− DCs, selectively take up dying cells and cross-present cell-associated antigens to naïve T cells. In this study, we identified genes that were more expressed in CD8α+ than CD8α− DCs by microarray analysis. Only one of these genes, when the extracellular domains were linked to human IgG Fc domain, could bind to late apoptotic or necrotic cells. This gene was a new member of the Triggering receptor expressed on myeloid cells (Trem) family, Trem-like 4 (Treml4). Treml4 mRNA and protein, the latter detected with a new monoclonal antibody, were predominantly expressed in spleen. Treml4, like other Trem family members, could associate with the adaptor molecule DAP12, but neither DAP10 nor FcRγ. Consistent with the microarray data, we confirmed that Treml4 protein was more expressed on CD8α+ than CD8 α− DCs, and we also found that Treml4 was expressed at high levels on splenic macrophages in spleen particularly red pulp and marginal metallophilic macrophages. In addition, Treml4 expression on DCs was not changed after maturation induced by Toll-like receptor ligands. Thus, Treml4 is a new Trem family molecule that is abundantly expressed on CD8α+ DCs and subsets of splenic resident macrophages, and can recognize dead cells by different types of phagocytes in spleen.
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影响因子：
30.5
作者：
Hamerman, JA;Tchao, NK;Lanier, LL
通讯作者：
Lanier, LL
DOI：
10.1084/jem.192.12.1685
发表时间：
2000-12-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
den Haan JM;Lehar SM;Bevan MJ
通讯作者：
Bevan MJ
影响因子：
4.4
作者：
Daws, MR;Sullam, PM;Seaman, WE
通讯作者：
Seaman, WE
影响因子：
64.8
作者：
Fadok, VA;Bratton, DL;Henson, PM
通讯作者：
Henson, PM
影响因子：
64.8
作者：
Bouchon, A;Facchetti, F;Colonna, M
通讯作者：
Colonna, M