High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins
High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins
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卟啉、叶绿素和胆碱的高效液相色谱和质谱分析
DOI：
10.1142/3411
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发表时间：
2009
影响因子：
--
通讯作者：
C. Lim
中科院分区：
文献类型：
--
作者：
C. Lim
介绍了卟啉和卟啉前体的高效液相色谱分离和定量方法。讨论了反相高效液相色谱法分离卟啉和卟啉原的理化背景、结构-保留关系及优化方法。有基于应用导向的例子，包括详细的说明和协议，从样品采集，处理和存储准备，以及HPLC分离和定量的实用技巧。描述了血红素生物合成酶活性的测量方法，并给出了不同类型卟啉症患者血液、尿液和粪便中卟啉的特征谱。还有关于卟啉的薄层色谱和毛细管电泳（CE）的章节。
Presents a compilation of HPLC methods for the separation and quantitation of porphyrins and porphyrin precursors. The physical-chemical background, structure-retention relationships and optimization of separation of porphyrins and porphyrinogens by reversed-phase HPLC are discussed. There are practical tips based on application-oriented examples, including detailed instructions and protocols, from sample collection, handling and storage preparation, and HPLC separation and quantitation. Methods for measurement of haem biosynthetic enzyme activities are described, and characteristic porphyrin profiles in the blood, urine and faeces of patients with different types of porphyrias are given. There are also chapters relating to TLC and capillary electrophoresis (CE) of porphyrins.