Comparison of antioxidant properties of different therapeutic albumin preparations
Comparison of antioxidant properties of different therapeutic albumin preparations
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DOI：
10.1016/j.biologicals.2016.04.002
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发表时间：
2016-07-01
期刊：
影响因子：
1.7
通讯作者：
Jorieux, Sylvie
中科院分区：
文献类型：
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作者：
Plantier, Jean-Luc;Duretz, Veronique;Jorieux, Sylvie
白蛋白对体内平衡有几个重要的作用，其中包括维持血浆氧化还原状态。本研究的目的是比较药物人血清白蛋白制剂的氧化还原状态，因为它反映了周围环境的氧化还原状态。使用一系列分析方法，比较了四种商用白蛋白的结构特征(钴离子结合、糖基化、分光光度和荧光图谱)以及它们清除羟基、过氧基或自由基的能力。不同的白蛋白表现出相似的结构特征以及羟基和过氧基清除活性。相比之下，来自LFB的白蛋白(Vialebex(R))具有显著更高的将电子转移到DPPH的能力，与其他与游离半胱氨酸-34水平相关的白蛋白相比。商业上可用的白蛋白的一些抗氧化特性有所不同。具有最高水平的游离半胱氨酸-34的白蛋白制剂显示出最高的抗氧化潜力。(C)2016 LFB BioTechnologies。爱思唯尔有限公司代表国际生物标准化联盟出版。
Albumin displays several important functions for homeostasis amongst which the maintenance of the plasma redox-state. The study aim was to compare the redox state of pharmaceutical human albumin preparations since it reflects the oxidation-reduction status of the surrounding environment. Using an array of analytical methods, four commercially available albumins were compared with respect to their structural characteristics (cobalt ion binding, glycation, spectrophotometric and fluorometric profiles) and their ability to scavenge hydroxyl, peroxyl or free radicals. The different albumins exhibited a similar structural profile as well as hydroxyl and peroxyl scavenging activities. By contrast, the albumin from LFB (Vialebex (R)) possessed a significantly higher capacity to transfer electrons to DPPH, as compared with other albumins that was correlated with the level of free cysteine-34.Commercially available albumins differed for some of their antioxidant properties. The albumin preparation possessing the highest level of free cysteine-34 exhibited the highest antioxidant potential. (C) 2016 LFB Biotechnologies. Published by Elsevier Ltd on behalf of International Alliance for Biological Standardization.