Plate-based assay for swimming motility in Pseudomonas aeruginosa.

Plate-based assay for swimming motility in Pseudomonas aeruginosa.
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DOI:
10.1007/978-1-4939-0473-0_7
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发表时间:
2014
期刊:
Methods in molecular biology (Clifton, N.J.)
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游泳运动是在革兰氏阴性细菌铜绿假单胞菌中观察到的鞭毛依赖性运动形式。游动运动性定义为在液体或低粘度条件下(琼脂浓度高达0.3%)的运动。与集群运动不同,游泳运动需要功能性鞭毛,但既不需要群体感应(QS)系统,也不需要生物表面活性剂。虽然游泳运动也可以通过显微镜观察,在这里,我们描述了一个可重复的板为基础的方法。
Swimming motility is a flagellum-dependent form of movement observed in the Gram-negative bacterium Pseudomonas aeruginosa. Swimming motility is defined as the movement in liquid or low-viscosity conditions (up to 0.3 % agar concentration). Unlike swarming motility, swimming motility requires a functional flagellum, but neither quorum sensing (QS) systems nor biosurfactants. While swimming motility can also be observed via microscopy, here we describe a reproducible plate-based method.