Plate-based assay for swimming motility in Pseudomonas aeruginosa.
Plate-based assay for swimming motility in Pseudomonas aeruginosa.
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DOI:
10.1007/978-1-4939-0473-0_7
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发表时间:
2014
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Swimming motility is a flagellum-dependent form of movement observed in the Gram-negative bacterium Pseudomonas aeruginosa. Swimming motility is defined as the movement in liquid or low-viscosity conditions (up to 0.3 % agar concentration). Unlike swarming motility, swimming motility requires a functional flagellum, but neither quorum sensing (QS) systems nor biosurfactants. While swimming motility can also be observed via microscopy, here we describe a reproducible plate-based method.