FUNCTIONAL ACTIVATION OF JAK1 AND JAK3 BY SELECTIVE ASSOCIATION WITH IL-2 RECEPTOR SUBUNITS
FUNCTIONAL ACTIVATION OF JAK1 AND JAK3 BY SELECTIVE ASSOCIATION WITH IL-2 RECEPTOR SUBUNITS
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DOI：
10.1126/science.7973659
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发表时间：
1994-11-11
期刊：
影响因子：
56.9
通讯作者：
TANIGUCHI, T
中科院分区：
文献类型：
--
作者：
MIYAZAKI, T;KAWAHARA, A;TANIGUCHI, T
白细胞介素2受体(IL-2)由三个亚基组成：IL-2Rα、IL-2Rβ和IL-2R伽马链，后者也用于IL-4、IL-7和IL-9的受体。IL-2刺激可诱导JAK1和JAK3的酪氨酸磷酸化和激活。JAK1和JAK3分别与IL-2Rβ的富丝氨酸区和IL-2Rγ的羧基末端区选择性相关。这两个区域都是IL-2信号传递所必需的。此外，在JAK3互补DNA的额外表达后，表达重组IL-2R的JAK3阴性成纤维细胞对IL-2有反应。因此，JAK1和JAK3的激活可能是IL-2信号转导中的一个关键事件。
The interleukin-2 receptor (IL-2) consists of three subunits: the IL-2R alpha, IL-2R beta, and IL-2R gamma chains, the last of which is also used in the receptors for IL-4, IL-7, and IL-9. Stimulation with IL-2 induces the tyrosine phosphorylation and activation of the Janus kinases Jak1 and Jak3. Jak1 and Jak3 were found to be selectively associated with the ''serine-rich'' region of IL-2R beta and the carboxyl-terminal region of IL-2R gamma, respectively. Both regions were necessary for IL-2 signaling. Furthermore, Jak3-negative fibroblasts expressing reconstituted IL-2R became responsive to IL-2 after the additional expression of Jak3 complementary DNA. Thus, activation of Jak1 and Jak3 may be a key event in IL-2 signaling.