Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction
Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction
复制标题
通过 Triton X-114 等温提取去除质粒样品中的内毒素
DOI：
10.1016/j.ab.2012.02.015
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发表时间：
2012-05-15
影响因子：
2.9
通讯作者：
Li, Li-Wen
中科院分区：
文献类型：
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作者：
Ma, Rui;Zhao, Jun;Li, Li-Wen
Triton X-114温度转换提取被认为是一种简单且具有成本效益的策略，用于从质粒制备物中消除内毒素。然而，反复的冷却-加热过程可能促进质粒DNA的降解。基于Triton X-114溶液的浊点在少量十二烷基硫酸钠（SDS）存在下显著增加以及电解质急剧降低Triton X-114-SDS溶液的浊点的发现，我们设计了一个Triton X 114等温提取法去除质粒样品中的内毒素，发现与温度过渡萃取法(c)2012 Elsevier Inc. All rights reserved.
Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling-heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud point of Triton X-114 solution increases substantially in the presence of small amounts of sodium dodecyl sulfate (SDS) and that electrolytes decrease the cloud point of Triton X-114-SDS solution drastically, we designed a Triton X-114 isothermal extraction method for removing endotoxin from plasmid samples and found that it has the same endotoxin removal efficiency when compared with the temperature transition extraction method. (c) 2012 Elsevier Inc. All rights reserved.