Carbohydrate binding properties of the stinging nettle (Urtica dioica) rhizome lectin.
Carbohydrate binding properties of the stinging nettle (Urtica dioica) rhizome lectin.
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荨麻（Urtica dioica）根茎凝集素的碳水化合物结合特性。
DOI：
10.1016/0003-9861(86)90577-1
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发表时间：
1986
影响因子：
3.9
通讯作者：
Broekaert,WF
中科院分区：
文献类型：
--
作者：
Shibuya,N;Goldstein,IJ;Shafer,JA;Peumans,WJ;Broekaert,WF
采用定量沉淀、半抗原抑制、平衡透析和紫外差光谱技术研究了荨麻根茎凝集素（UDA）与碳水化合物的相互作用。 UDA 的碳水化合物结合位点被确定为与 N,N',N''-三乙酰基壳三糖单元互补，并建议由三个亚位点组成，每个亚位点的结合特异性略有不同。 UDA 还具有与碳水化合物结合位点相邻的疏水相互作用区域。平衡透析和紫外差异光谱显示，UDA 每个由单个多肽链组成的分子有两个碳水化合物结合位点。这些结合位点对于配体分子具有本质上不同的亲和力，或者它们可能对配体结合表现出负协同性。
The interaction of the stinging nettle rhizome lectin (UDA) with carbohydrates was studied by using the techniques of quantitative precipitation, hapten inhibition, equilibrium dialysis, and uv difference spectroscopy. The Carbohydrate binding site of UDA was determined to be complementary to anN,N′,N″-triacetylchitotriose unit and proposed to consist of three subsites, each of which has a slightly different binding specificity. UDA also has a hydrophobic interacting region adjacent to the carbohydrate binding site. Equilibrium dialysis and uv difference spectroscopy revealed that UDA has two carbohydrate binding sites per molecule consisting of a single polypeptide chain. These binding sites either have intrinsically different affinities for ligand molecules, or they may display negative cooperativity toward ligand binding.