Temporal and spatial expression of TGF-beta1 in an Achilles tendon section model after application of platelet-rich plasma.
Temporal and spatial expression of TGF-beta1 in an Achilles tendon section model after application of platelet-rich plasma.
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DOI：
10.1016/j.fas.2009.09.002
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发表时间：
2010-09-01
期刊：
影响因子：
--
通讯作者：
Verettas, Dionysios
中科院分区：
文献类型：
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作者：
Lyras, Dimitrios N;Kazakos, Konstantinos;Verettas, Dionysios
背景技术背景：研究富血小板血浆（PRP）对肌腱愈合过程中TGF-β 1表达的影响。24只家兔接受PRP，24只家兔作为未处理对照组。在第1、2、3和4周处死相同数量的动物。手术过程涉及横切跟腱的横向切口。PRP组在肌腱内注射PRP 1 ml。组织学和免疫组织化学评价与抗TGF-β的初级antibody performed.RESULTS：在PRP组的TGF-β 1的表达模式的特点是一个显着上调在第一个2周和随后显着下调在第3和第4周与对照组相比。我们的研究结果表明，PRP可能会影响肌腱愈合过程中通过改变TGF-β 1的表达。
BACKGROUND: To investigate the effect of platelet-rich plasma (PRP) on TGF-beta1 expression during tendon healing.METHODS: We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-beta primary antibody were performed.RESULTS: The pattern of expression of TGF-beta1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls.CONCLUSIONS: Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-beta1.