A carrot G-box binding factor-type basic region/leucine zipper factor DcBZ1 is involved in abscisic acid signal transduction in somatic embryogenesis
A carrot G-box binding factor-type basic region/leucine zipper factor DcBZ1 is involved in abscisic acid signal transduction in somatic embryogenesis
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DOI：
10.1016/j.plaphy.2008.02.010
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发表时间：
2008-05-01
影响因子：
6.5
通讯作者：
Kamada, Hiroshi
中科院分区：
文献类型：
--
作者：
Shiota, Hajime;Ko, Sukmin;Kamada, Hiroshi
胡萝卜（Daucus carota）体细胞胚胎发生已被广泛用作研究胚胎发生的实验系统。在成熟的合子胚胎中，脱落酸 (ABA) 参与干燥耐受性和休眠的获得。另一方面，体细胞胚含有低水平的内源ABA，表现出不耐干燥性和缺乏休眠性，但外源ABA的应用可以诱导耐受性和休眠性。在 ABA 处理的胡萝卜胚胎中，一些 ABA 诱导基因得到表达。我们从胡萝卜体细胞胚胎中分离出了编码 G 盒结合因子型碱性区/亮氨酸拉链（GBF 型 bZIP）因子的胡萝卜 bZIP1（DcBZ1）基因。在胚胎发生细胞、非胚胎发生细胞、体细胞胚胎、发育中的种子、幼苗和真叶中检测到DcBZ1的表达。值得注意的是，在胚胎细胞、真叶和幼苗中检测到较高的表达。 ABA处理后，DcBZ1在幼苗和真叶中的表达增加，而表达不受光照条件差异的影响。在合子和体细胞胚胎的发育过程中，通常在发育后期检测到 DcBZ1 表达增加。根据电泳迁移率变动测定的结果，重组 DcBZ1 蛋白显示出与胡萝卜 ABA 诱导基因启动子区域中的两个 ABA 响应元件样基序（基序 X 和基序 Y）的特异性结合活性。我们的研究结果表明，胡萝卜 GBF 型 bZIP 因子 DcBZ1 参与胚胎发生和其他营养组织中的 ABA 信号转导。 (C) 2008 Elsevier Masson SAS。版权所有。
Carrot (Daucus carota) somatic embryogenesis has been extensively used as an experimental system for studying embryogenesis. In maturing zygotic embryos, abscisic acid (ABA) is involved in acquisition of desiccation tolerance and dormancy. On the other hand, somatic embryos contain low levels of endogenous ABA and show desiccation intolerance and lack dormancy, but tolerance and dormancy can be induced by exogenous application of ABA. In ABA-treated carrot embryos, some ABA-inducible genes are expressed. We isolated the Daucus carota bZIP1 (DcBZ1) gene encoding a G-box binding factor-type basic region/leucine zipper (GBF-type bZIP) factor from carrot somatic embryos. The expression of DcBZ1 was detected in embryogenic cells, non-embryogenic cells, somatic embryos, developing seeds, seedlings, and true leaves. Notably, higher expression was detected in embryogenic cells, true leaves, and seedlings. The expression of DcBZ1 increased in seedlings and true leaves after ABA treatment, whereas expression was not affected by differences in light conditions. During the development of zygotic and somatic embryos, increased expression of DcBZ1 was commonly detected in the later phase of development. The recombinant DcBZ1 protein showed specific binding activity to the two ABA-responsive element-like motifs (motif X and motif Y) in the promoter region of the carrot ABA-inducible gene according to results from an electrophoretic mobility shift assay. Our findings suggest that the carrot GBF-type bZIP factor, DcBZ1, is involved in ABA signal transduction in embryogenesis and other vegetative tissues. (C) 2008 Elsevier Masson SAS. All rights reserved.