Terapixel hyperspectral whole-slide imaging via slit-array detection and projection
Terapixel hyperspectral whole-slide imaging via slit-array detection and projection
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DOI：
10.1117/1.jbo.23.6.066503
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发表时间：
2018-06-01
影响因子：
3.5
通讯作者：
Zheng, Guoan
中科院分区：
文献类型：
--
作者：
Liao, Jun;Jiang, Shaowei;Zheng, Guoan
通过全幻灯片成像(WSI)系统的数字病理学最近已被批准用于美国的主要诊断用途。获取在每个像素具有光谱信息的全载玻片图像允许使用多路抗体标记，并允许测量细胞分辨的化学信息。在这里，我们报告了一种使用棱镜狭缝阵列色散的高通量太像素高光谱WSI系统的发展。我们演示了用于基于吸收的测量的狭缝阵列检测方案和用于基于荧光的测量的狭缝阵列投影方案。在所报道的方案中，光谱分辨率和光谱范围可以通过改变狭缝阵列掩模的取向来调整。用我们的系统采集了74幅500万像素的不同波长的亮场图像，相当于1张S的图像，相当于0.375 GB像素/S的吞吐量，在类似45分钟的时间内就可以获得一个太像素的全幻灯片空间光谱数据立方体。报告的系统与现有的WSI系统兼容，可以开发为全幻灯片光谱成像的附加模块。它可能在高通量多抗体标记的化学成像中有广泛的应用。将缝隙阵列用于结构照明也可以为开发高通量高光谱共焦成像系统提供见解。(C)2018年光学仪器工程师学会(SPIE)
Digital pathology via whole-slide imaging (WSI) systems has recently been approved for the primary diagnostic use in the US. Acquiring whole-slide images with spectral information at each pixel permits the use of multiplexed antibody labeling and allow for the measurement of cellularly resolved chemical information. Here, we report the development of a high-throughput terapixel hyperspectral WSI system using prism-based slit-array dispersion. We demonstrate a slit-array detection scheme for absorption-based measurements and a slit-array projection scheme for fluorescence-based measurements. The spectral resolution and spectral range in the reported schemes can be adjusted by changing the orientation of the slit-array mask. We use our system to acquire 74 5-megapixel brightfield images at different wavelengths in similar to 1 s, corresponding to a throughput of 0.375 gigapixels/s. A terapixel whole-slide spatial-spectral data cube can be obtained in similar to 45 min. The reported system is compatible with existing WSI systems and can be developed as an add-on module for whole-slide spectral imaging. It may find broad applications in high-throughput chemical imaging with multiple antibody labeling. The use of slit array for structured illumination may also provide insights for developing high-throughput hyperspectral confocal imaging systems. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)