Leaf chlorophyll, net gas exchange and chloroplast ultrastructure in citrus leaves of different nitrogen status
Leaf chlorophyll, net gas exchange and chloroplast ultrastructure in citrus leaves of different nitrogen status
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DOI：
10.1093/treephys/23.8.553
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发表时间：
2003-06-01
期刊：
影响因子：
4
通讯作者：
Syvertsen, JP
中科院分区：
文献类型：
--
作者：
Bondada, BR;Syvertsen, JP
在温室中对1年生的红橘（Citrus reticulata布兰科）幼苗进行了4种氮肥施用频率的研究。缺氮（86 mmol Nm（-2））叶片单位面积叶绿素含量较低，但叶绿素a：B比高于施氮（> 187 mmol Nm（-2））。叶片单位面积干重（DM面积（-1））和叶绿素总浓度随叶片氮浓度的增加而线性增加，叶绿素a：B比值则呈下降趋势。CO2净同化量（A（CO2））和叶片水分利用效率（WUE）均在叶片中达到最大值，接近187 mmolNm（-2）。缺氮叶片表现出小的叶绿体，没有淀粉粒;基粒和基质层片，这与许多大plastastelluli在基质中的增生一致解体。高氮叶片叶绿体大，基粒发达，基质片层和淀粉粒随氮浓度增加而增大。当叶片氮浓度高于187 mmol N m（-2）时，A（CO2）容量没有增加，这似乎与大量大淀粉颗粒的存在有关。
One-year-old 'Cleopatra mandarin' (Citrus reticulata Blanco) seedlings were raised in a greenhouse and fertilized with nitrogen (N) at four application frequencies. Nitrogen-deficient leaves (86 mmol N m(-2)) had less chlorophyll per unit area, but a greater chlorophyll a:b ratio than N-fertilized leaves (> 187 mmol N m(-2)). Leaf dry mass per area (DM area(-1)) and total chlorophyll concentration increased linearly with increasing leaf N, whereas chlorophyll a:b ratio declined. Net assimilation of CO2 (A(CO2)) and leaf water-use efficiency (WUE) reached maximum values in leaves with similar to187 mmol N m(-2). Nitrogen-deficient leaves exhibited small chloroplasts with no starch granules; grana and stroma lamellae that coincided with the accretion of numerous large plastoglobuli in the stroma disintegrated. High-N leaves had large chloroplasts with well-developed grana, stroma lamellae and starch granules that enlarged with increasing N concentration. The lack of an increase in A(CO2) capacity at leaf N concentrations above 187 mmol N m(-2) appeared to be correlated with the presence of numerous large starch granules.