Molecular Identification and Genetic Diversity of Fusarium species Associated with Onion Fields in Turkey
Molecular Identification and Genetic Diversity of Fusarium species Associated with Onion Fields in Turkey
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DOI：
10.1111/j.1439-0434.2010.01715.x
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发表时间：
2011-01-01
影响因子：
1.5
通讯作者：
Dolar, Fatma Sara
中科院分区：
文献类型：
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作者：
Bayraktar, Harun;Dolar, Fatma Sara
为了鉴定与洋葱根部和基盘病害有关的镰刀菌种类，2007年在土耳其的7个省进行了调查。对223个田间进行了采样，共分离到7个镰刀菌属的332个菌株。得到了。经形态和培养特征鉴定为尖孢镰刀菌、立枯镰刀菌、尖孢镰刀菌、木霉、增殖镰刀菌、雷氏镰刀菌和球孢镰刀菌。此外，还利用种属特异性引物对镰刀菌种进行了鉴定。尖孢镰刀菌是最常见的病原菌，占镰刀菌总种数的66.57%。在土耳其的洋葱种植区首次发现了红曲霉。对筛选出的每种菌株对洋葱植株的侵袭性进行了评价。尖孢镰刀菌、立枯镰刀菌、尖孢镰刀菌、增殖镰刀菌和红镰刀菌对洋葱植株有较强的致病性，可引起洋葱严重的立枯病。德克萨斯州早期格拉诺。ISSR标记揭示了镰刀菌种内和种间高度的多态现象。
To identify Fusarium species associated with diseases of root and basal plate of onion, surveys were conducted in seven provinces of Turkey in 2007. Samplings were performed in 223 fields, and 332 isolates belonging to 7 Fusarium spp. were obtained. The isolates were identified as F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. proliferatum, F. redolens, and F. culmorum based on morphological and cultural characteristics. Also, species-specific primers were used to confirm the identity of Fusarium species. F. oxysporum was the most commonly isolated species, comprising 66.57% of the total Fusarium species. F. redolens was identified for the first time in onion-growing areas of Turkey. Selected isolates of each species were evaluated for their aggressiveness on onion plant. F. oxysporum, F. solani, F. acuminatum, F. proliferatum, and F. redolens were highly pathogenic, causing severe damping-off on onion plants cv. Texas Early Grano. Inter-simple sequence repeats (ISSR) markers revealed a high degree of intra- and interspecific polymorphisms among Fusarium spp.