Identification of loci essential for the growth of Helicobacter pylori under acidic conditions
Identification of loci essential for the growth of Helicobacter pylori under acidic conditions
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DOI：
10.1086/315855
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发表时间：
2000-11-01
影响因子：
6.4
通讯作者：
Kusters, JG
中科院分区：
文献类型：
--
作者：
Bijlsma, JJE;Lie-A-Ling, M;Kusters, JG
为了在胃的酸性环境中存活，幽门螺杆菌必须在酸休克中存活并在酸性pH下生长。在筛选的1250个随机突变体的文库中筛选出不能在低pH下生长的分离株，检测到10个突变体不能在pH 4，8下生长，然而，所有10个突变体都对酸休克有抗性，4个突变体在未知功能的基因中插入，一个突变体在lepA中受到影响，lepA是一种膜GT酶的直向同源物，三个突变体在涉及HC离子或其他阳离子转运的位点（FRase I，czcA和醛酮还原酶）中受到破坏，两个突变体在其他微生物中有助于耐酸性的位点（uvrA和atpF）中受到影响。因此，至少有10个与脲酶无关的位点对H.幽门螺杆菌在酸性条件下，应该是由这种病原体终身感染的关键。
To persist in the hostile acidic environment of the stomach, Helicobacter pylori must survive acid shock and grow at acidic pH, Of a library of 1250 random mutants screened for isolates unable to grow at low pH, 10 mutants were detected that were unable to grow at pH 4,8, However, all 10 mutants were resistant to acid shock, Four mutants had an insertion in genes of unknown function, One mutant was affected in lepA, an orthologue of a membrane GTPase, Three mutants were disrupted in loci involved in the transport of HC ions or other cations (FRaseI, czcA, and aldo-keto reductase), Two mutants were affected in loci that contribute to acid resistance in other microorganisms (uvrA and atpF). Thus, at least 10 loci not related to urease are essential for the growth of H. pylori under acidic conditions and should be critical for lifelong infection by this pathogen.