Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay.
Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay.
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使用 Miles 测定评估 VEGF 诱导的血管渗漏。
DOI：
10.1007/978-1-0716-2217-9_21
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Brash JT
中科院分区：
文献类型：
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作者：
Brash JT
在描述血管内皮生长因子A（VEGF）的内皮促有丝分裂活性之前，已经鉴定了VEGF诱导血管渗漏的能力。VEGF诱导的血管渗漏最常使用Miles测定法在体内研究，Miles测定法是一种简单而宝贵的技术，它使研究人员能够解开VEGF和其他渗透性诱导剂的血管渗漏的分子机制。在该方案中，在通过皮内注射局部施用VEGF之前，向小鼠静脉内注射伊文思蓝染料。VEGF促进真皮中血清蛋白的血管渗漏，使得伊文思蓝标记的白蛋白从循环中外渗并随后在皮肤中积累。由于染料外渗的体积与血管渗漏的程度成比例，因此可以将其量化为VEGF诱导的血管渗漏的替代测量。
Before the endothelial mitogenic activity of the Vascular Endothelial Growth Factor A (VEGF) was described, VEGF had already been identified for its ability to induce vascular leakage. VEGF-induced vascular leakage has been most frequently studied in vivo using the Miles assay, a simple yet invaluable technique that has allowed researchers to unravel the molecular mechanisms underpinning vascular leakage both for VEGF and other permeability inducing agents. In this protocol, a mouse is intravenously injected with Evans Blue dye before VEGF is administered locally via intradermal injection. VEGF promotes vascular leak of serum proteins in the dermis, enabling Evans Blue-labeled albumin extravasation from the circulation and subsequent accumulation in the skin. As the volume of dye extravasation is proportional to the degree of vascular leak, it can be quantified as a proxy measurement of VEGF-induced vascular leakage.