Isolation of decidual lymphocytes from chorionic villus samples: phenotypic analysis and growth in vitro.
Isolation of decidual lymphocytes from chorionic villus samples: phenotypic analysis and growth in vitro.
复制标题
从绒毛膜绒毛样品中分离蜕膜淋巴细胞：表型分析和体外生长。
DOI：
10.1111/j.1600-0897.1995.tb00884.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Smith,JB
中科院分区：
文献类型：
--
作者：
Haynes,MK;Flanagan,MT;Perussia,B;Jackson,LG;Smith,JB
从绒毛标本中分离的Giemsa染色细胞含有形态类似于大颗粒淋巴细胞的颗粒状细胞。方法：采用双色免疫荧光技术对分离的细胞进行表型鉴定，并在重组白介素2(rIL-2)的存在下进行体外培养，以确定CVS是否可以作为蜕膜淋巴细胞的来源。结果：CVS来源的淋巴细胞大部分为蜕膜NK细胞，CD56+Bright高表达，CD16不表达。与外周血相比，CVS来源的淋巴细胞中存在的T细胞群中同时含有CD4+和CD8+细胞的比例有所降低。结论：CVS中含有蜕膜NK细胞和T细胞，CD56+bnght细胞在rIL-2和/或第三方刺激细胞刺激下增殖。这些培养技术将使研究蜕膜NK细胞和T细胞的成熟和/或激活成为可能。
PROBLEM: Giemsa stained cell isolates prepared from chorionic villus samples (CVS) contain granulated cells morphologically similar to large granular lymphocytes.METHOD: Phenotypic characterization of these cellular isolates by two‐color immunofluorescence and subsequent in vitro culture in the presence of recombinant interleukin‐2 (rIL‐2) were done in order to determine whether CVS could serve as a source of decidual lymphocytes.RESULTS: A major fraction of the CVS‐derived lymphocytes were characterized as decidual NK cells, exhibiting high levels of CD56 expression (CD56+bright), without concomitant expression of CD16. The T cell population present in CVS‐derived lymphocytes contained both CD4+and CD8+cells in a ratio somewhat reduced compared to that found in peripheral blood. While both T cells and CD56+brightcells from CVS proliferate in vitro in response to rIL‐2 alone, preferential growth of CD56+bnghtcells was accomplished using a selective culture technique wherein co‐culture with an irradiated, B lymphoblastoid cell line promoted the growth of CD56+cells.CONCLUSION: CVS contains decidual NK cells and T cells that proliferate in response to rIL‐2 and/or third party stimulator cells. These culture techniques will allow investigations into the maturation and/or activation of decidual NK cells and T cells.