Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.).
Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.).
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DOI：
10.1016/j.ygeno.2011.04.007
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发表时间：
2011-07
期刊：
影响因子：
4.4
通讯作者：
Dongxia Yao;Xueyang Zhang;Xinhua Zhao;Chuanliang Liu;Chun-Chao Wang;Zhenghai Zhang;Chao-jun Zhang
中科院分区：
文献类型：
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作者：
Dongxia Yao;Xueyang Zhang;Xinhua Zhao;Chuanliang Liu;Chun-Chao Wang;Zhenghai Zhang;Chao-jun Zhang
高盐是限制棉花生长和产量的主要因素之一。以TM-1高地棉为材料，采用基因芯片和实时荧光定量PCR（RT-PCR）技术对盐胁迫下盐胁迫相关基因进行了检测。基因芯片分析表明，在100 mM NaCl胁迫3 h的植株中，有1503个探针组表达上调，1490个探针组表达下调，RT-PCR分析验证了42个相关基因。丰富的基因本体论术语的分布显示了NaCl处理诱导的响应水分胁迫、激素代谢和信号转导途径等重要过程。WRKY、ERF和JAZ等与非生物胁迫和生物胁迫相关的关键调控基因家族的差异表达。我们的转录组分析可能提供一些有用的见解盐介导的信号转导途径在棉花和提供一些候选基因作为耐盐胁迫的潜在标记。
High salinity is one of the main factors limiting cotton growth and productivity. The genes that regulate salt stress in TM-1 upland cotton were monitored using microarray and real-time PCR (RT-PCR) with samples taken from roots. Microarray analysis showed that 1503 probe sets were up-regulated and 1490 probe sets were down-regulated in plants exposed for 3 h to 100 mM NaCl, and RT-PCR analysis validated 42 relevant/related genes. The distribution of enriched gene ontology terms showed such important processes as the response to water stress and pathways of hormone metabolism and signal transduction were induced by the NaCl treatment. Some key regulatory gene families involved in abiotic and biotic sources of stress such as WRKY, ERF, and JAZ were differentially expressed. Our transcriptome analysis might provide some useful insights into salt-mediated signal transduction pathways in cotton and offer a number of candidate genes as potential markers of tolerance to salt stress.