Microinjected glutathione S-transferase Yb subunits translocate to the cell nucleus.
Microinjected glutathione S-transferase Yb subunits translocate to the cell nucleus.
复制标题
显微注射的谷胱甘肽 S-转移酶 Yb 亚基易位至细胞核。
DOI：
10.1042/bj2470109
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Yeoman,LC
中科院分区：
文献类型：
--
作者：
Bennett,CF;Yeoman,LC
我们之前已经证明，从大鼠细胞核中分离出的 30 kDa DNA 结合蛋白表现出谷胱甘肽 S-转移酶 Yb 亚基的化学和免疫学特性 [Bennett, Spector & Yeoman (1986) J. Cell Biol. 102、600-609]。因此，确定从大鼠肝细胞核中分离的 Yb 亚基在通过红细胞介导的融合重新引入细胞质后是否会返回到核部分是很有意义的。细胞融合后 60 分钟，标记的 Yb 亚基与核级分相关。显微注射的蛋白质与细胞核保持结合 18 小时，并且不能用低盐洗涤提取。此外，发现注入的 Yb 亚基均匀分布在可提取核部分 (56%) 和残留核部分 (44%) 之间。这些实验证明，从细胞核中分离的谷胱甘肽S-转移酶Yb亚基在重新引入细胞质后迅速易位至细胞核。
We have previously shown that a 30 kDa DNA-binding protein isolated from rat cell nuclei exhibits the chemical and immunological properties of glutathione S-transferase Yb subunits [Bennett, Spector & Yeoman (1986) J. Cell Biol. 102, 600-609]. It was of interest, therefore, to determine whether Yb subunits isolated from rat liver nuclei would return to nuclear fractions upon reintroduction to cell cytoplasms via red-blood-cell-mediated fusion. Labelled Yb subunits were associated with nuclear fractions 60 min after cell fusion. The microinjected protein remained associated with the nuclei for 18 h and was not extractable with low-salt washes. In addition, injected Yb subunits were found to equally distribute between extractable (56%) and residual (44%) nuclear fractions. These experiments demonstrate that glutathione S-transferase Yb subunits isolated from nuclei rapidly translocate to nuclei upon reintroduction into cell cytoplasms.