Electroactive self-assembled monolayers that permit orthogonal control over the adhesion of cells to patterned substrates
Electroactive self-assembled monolayers that permit orthogonal control over the adhesion of cells to patterned substrates
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DOI：
10.1021/la061212y
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发表时间：
2006-12-05
期刊：
影响因子：
3.9
通讯作者：
Mrksich, Milan
中科院分区：
文献类型：
--
作者：
Yeo, Woon-Seok;Mrksich, Milan
本文介绍了一种电活性基板，显示两个独立的动态功能，用于控制细胞的粘附。该方法是基于自组装单分子膜的黄金图案的区域提出的Arg-Gly-Asp肽细胞粘附配体。图案化区域的不同之处在于将肽系在单层上的接头的电化学性质。在这项工作中，采用了三种不同的化学物质，其提供了在施加负电势时释放配体，在施加正电势时释放配体，以及响应于电势没有变化。使细胞附着到图案化成包含三种化学物质的圆形区域的单层上。用650或-650 mV的电势处理导致仅从显示相关电活性基团的区域选择性释放粘附细胞。该实施例建立了具有多种功能的动态底物的制备，并且对于制备源自多种细胞类型的模型培养物是重要的，控制每个细胞群体的时间相互作用。
This article describes an electroactive substrate that displays two independent dynamic functions for controlling the adhesion of cells. The approach is based on self-assembled monolayers on gold that are patterned into regions presenting the Arg-Gly-Asp peptide cell adhesion ligand. The patterned regions differ in the electrochemical properties of the linkers that tether the peptides to the monolayer. In this work, three distinct chemistries are employed that provide for release of the ligand on application of a negative potential, release of the ligand on application of a positive potential, and no change in response to a potential. Cells were allowed to attach to a monolayer patterned into circular regions comprising the three chemistries. Treatment with electric potentials of 650 or- 650 mV resulted in the selective release of adherent cells only from regions that display the relevant electroactive groups. This example establishes the preparation of dynamic substrates with multiple functions and will be important to preparing model cultures derived from multiple cell types, with control over the temporal interactions of each cell population.