Bioethanol production from tension and opposite wood of Eucalyptus globulus using organosolv pretreatment and simultaneous saccharification and fermentation
Bioethanol production from tension and opposite wood of Eucalyptus globulus using organosolv pretreatment and simultaneous saccharification and fermentation
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DOI：
10.1007/s10295-011-0975-y
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发表时间：
2011-11-01
影响因子：
3.4
通讯作者：
Teixeira Mendonca, Regis
中科院分区：
文献类型：
--
作者：
Munoz, Claudio;Baeza, Jaime;Teixeira Mendonca, Regis
在树木的生长过程中，硬木可以启动张力木材的形成，这是一种在树干和树枝的上部受到强烈压力的木材。在蓝桉中，与对生材相比，张紧材表现出更宽、更厚的细胞壁，木质素较低，葡聚糖相似，木聚糖含量较高。在这项工作中，分离和评价了以乙醇/水脱木素为前处理，同时糖化发酵(SSF)生产生物乙醇的蓝桉树的张力和对生材。在相同的反应H因子下，张力木有机溶剂浆的木素残留量较低，葡聚糖保留率较高，而相反木浆的残留率较低。较快的脱木素速度与抗张木素含量较低有关，比对生材低15%。在低H因子和高H因子(分别为3,900和12,500)条件下获得的Organosolv浆经纤维素酶糖化后，葡聚糖的转化率分别达到69%和77%。考虑到发酵过程中葡萄糖转化为乙醇的51%，SSF的生物乙醇产量高达35g/L，相当于木材最大理论产量的85%-95%，与以往有机溶胶浆从阔叶木转化为生物乙醇的研究相比，这是一个非常令人满意的结果。蓝桉的张力材和对生材都是有机溶剂前处理和生物乙醇生产的适宜原料，转化率高。
During tree growth, hardwoods can initiate the formation of tension wood, which is a strongly stressed wood on the upper side of the stem and branches. In Eucalyptus globulus, tension wood presents wider and thicker cell walls with low lignin, similar glucan and high xylan content, as compared to opposite wood. In this work, tension and opposite wood of E. globulus trees were separated and evaluated for the production of bioethanol using ethanol/water delignification as pretreatment followed by simultaneous saccharification and fermentation (SSF). Low residual lignin and high glucan retention was obtained in organosolv pulps of tension wood as compared to pulps from opposite wood at the same H-factor of reaction. The faster delignification was associated with the low lignin content in tension wood, which was 15% lower than in opposite wood. Organosolv pulps obtained at low and high H-factor (3,900 and 12,500, respectively) were saccharified by cellulases resulting in glucan-to-glucose yields up to 69 and 77%, respectively. SSF of the pulps resulted in bioethanol yields up to 35 g/l that corresponded to 85-95% of the maximum theoretical yield on wood basis, considering 51% the yield of glucose to ethanol conversion in fermentation, which could be considered a very satisfactory result compared to previous studies on the conversion of organosolv pulps from hardwoods to bioethanol. Both tension and opposite wood of E. globulus were suitable raw materials for organosolv pretreatment and bioethanol production with high conversion yields.