Wheat Bran Does Not Affect Postprandial Plasma Short-Chain Fatty Acids from 13C-inulin Fermentation in Healthy Subjects
Wheat Bran Does Not Affect Postprandial Plasma Short-Chain Fatty Acids from 13C-inulin Fermentation in Healthy Subjects
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DOI：
10.3390/nu9010083
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发表时间：
2017-01-01
期刊：
影响因子：
5.9
通讯作者：
Verbeke, Kristin
中科院分区：
文献类型：
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作者：
Deroover, Lise;Verspreet, Joran;Verbeke, Kristin
小麦麸皮是全谷物产品的重要组成部分，对人体健康有重要作用。在人类结肠内，这种不溶性颗粒可能被特定的微生物群体定殖，这些微生物群体可以刺激交叉喂养，导致更有效的碳水化合物发酵和增加的丁酸盐产量。我们研究了具有不同性质的WB级分影响结肠中其他碳水化合物发酵的程度。十名健康受试者进行了四个测试日，在此期间他们食用了补充有10 g C-13-菊糖的标准早餐。还将总共20 g的WB级分（未改性的WB、具有减小的粒度的麦麸（WB RPS）或脱淀粉的富含果皮的麦麸（PE WB））添加到早餐中，除了一个测试日，其用作对照。在14 h的定期时间点采集血样，以测量C-13标记的短链脂肪酸（SCFA;乙酸盐、丙酸盐和丁酸盐）浓度。C-13-菊糖发酵导致血浆SCFA增加约8小时，表明通过给予中等剂量的碳水化合物，每天三次，可以实现血浆SCFA的持续增加。然而，添加单剂量WB组分并未进一步增加血浆中的C-13-SCFA浓度，也未刺激交叉喂养（Wilcoxon符号秩检验）。
Wheat bran (WB) is a constituent of whole grain products with beneficial effects for human health. Within the human colon, such insoluble particles may be colonized by specific microbial teams which can stimulate cross-feeding, leading to a more efficient carbohydrate fermentation and an increased butyrate production. We investigated the extent to which WB fractions with different properties affect the fermentation of other carbohydrates in the colon. Ten healthy subjects performed four test days, during which they consumed a standard breakfast supplemented with 10 g C-13-inulin. A total of 20 g of a WB fraction (unmodified WB, wheat bran with a reduced particle size (WB RPS), or de-starched pericarp-enriched wheat bran (PE WB)) was also added to the breakfast, except for one test day, which served as a control. Blood samples were collected at regular time points for 14 h, in order to measure C-13-labeled short-chain fatty acid (SCFA; acetate, propionate and butyrate) concentrations. Fermentation of C-13-inulin resulted in increased plasma SCFA for about 8 h, suggesting that a sustained increase in plasma SCFA can be achieved by administering a moderate dose of carbohydrates, three times per day. However, the addition of a single dose of a WB fraction did not further increase the C-13-SCFA concentrations in plasma, nor did it stimulate cross-feeding (Wilcoxon signed ranks test).