The root microtubule cytoskeleton and cell cycle analysis through desiccation of Brassica napus seedlings
The root microtubule cytoskeleton and cell cycle analysis through desiccation of Brassica napus seedlings
复制标题
DOI：
10.1007/s00709-008-0001-z
复制
发表时间：
2008-11-01
期刊：
影响因子：
2.9
通讯作者：
Bagniewska-Zadworna, Agnieszka
中科院分区：
文献类型：
--
作者：
Bagniewska-Zadworna, Agnieszka
传统种子的干燥耐受性（DT）在发芽时会降低。本研究的主要目的是通过检查干燥对不同细胞中微管的分布、稳定性和方向的影响来估计油菜幼苗 DT 的范围。使用不同的参数，例如相对水含量（RWC）、四唑活性测试和电解质渗漏，已经证明相对湿度的小百分比下降会导致膜通透性以及核结构和微管细胞骨架稳定性发生不可挽回的变化。当暴露于低干燥胁迫强度时，幼苗根尖存活，但观察到微管行为的微小变化。皮质微管形成厚阵列，尤其是在质膜附近。水分流失还导致有丝分裂活性降低。更快速的干燥导致微管解聚。偶尔可以看到异常的微管蛋白聚集体。在这些条件下无法检测到细胞分裂。由于可观察到的微管缺陷，微管细胞骨架的超敏性可能是估计环境应激强度的有用且简单的参数。
Desiccation tolerance (DT) of orthodox seeds is reduced upon their germination. The main aim of this study was to estimate the range of rape seedling DT by examining the consequences of desiccation on the distribution, stability and orientation of microtubules in diverse cells. Using different parameters, such as relative water content (RWC), the tetrazolium viability test and electrolyte leakage, it has been demonstrated that a small percentage decrease in relative humidity can cause irreparable changes in membrane permeability, as well as in nuclear structure and microtubule cytoskeleton stability. Seedling root tips survived when exposed to low desiccation stress intensity, but small changes in microtubule behavior were observed. Cortical microtubules formed thick arrays, especially near the plasma membrane. Water loss also resulted in a reduction of the mitotic activity. More rapid desiccation caused microtubule depolymerization. Occasionally, abnormal tubulin aggregates were visible. Cell divisions were not detectable under these conditions. Due to the observable microtubule defects, the hypersensitivity of the microtubule cytoskeleton might be a useful and simple parameter for estimating environmental stress intensity.