INTERLEUKIN-2 ACTIVATION OF NATURAL-KILLER CELLS RAPIDLY INDUCES THE EXPRESSION AND PHOSPHORYLATION OF THE LEU-23 ACTIVATION ANTIGEN
INTERLEUKIN-2 ACTIVATION OF NATURAL-KILLER CELLS RAPIDLY INDUCES THE EXPRESSION AND PHOSPHORYLATION OF THE LEU-23 ACTIVATION ANTIGEN
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DOI：
10.1084/jem.167.5.1572
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发表时间：
1988-05-01
影响因子：
15.3
通讯作者：
PHILLIPS, JH
中科院分区：
文献类型：
--
作者：
LANIER, LL;BUCK, DW;PHILLIPS, JH
IL-2增强T淋巴细胞和NK细胞的生长和细胞毒性功能。静息的外周血NK细胞可以直接对rIL-2产生反应，而不需要辅助细胞或辅助因子，并且可以在暴露于这种淋巴因子后几小时内测量到增强的细胞毒性。在这项研究中，我们描述了一种活化抗原，Leu-23，这是迅速诱导和磷酸化后，IL-2刺激的NK细胞和一个子集的低浮力密度T淋巴细胞。以前，一直不确定是否所有NK细胞或仅一个子集对IL-2有反应。由于在暴露于IL-2后18小时内，基本上所有NK细胞都表达Leu-23，这些发现表明所有外周血NK细胞都对IL-2的刺激有反应。Leu-23抗原是二硫键连接的同源二聚体，由24 kD蛋白亚基和两个N-连接的寡糖组成。这种糖蛋白在NK细胞上的出现是IL-2依赖性的，并且与IL-2诱导的针对NK耐药实体瘤细胞靶标的细胞毒性密切平行。
IL-2 potentiates both growth and cytotoxic function of T lymphocytes and NK cells. Resting peripheral blood NK cells can respond directly to rIL-2, without requirement for accessory cells or cofactors, and enhanced cytotoxicity can be measured within a few hours after exposure to this lymphokine. In this study, we describe an activation antigen, Leu-23, that is rapidly induced and phosphorylated after IL-2 stimulation of NK cells and a subset of low buoyant density T lymphocytes. Previously, it has been uncertain whether all NK cells or only a subset are responsive to IL-2. Since within 18 h after exposure to IL-2, essentially all NK cells express Leu-23, these findings indicate that all peripheral blood NK cells are responsive to stimulation by IL-2. The Leu-23 antigen is a disulfide-bonded homodimer, composed of 24-kD protein subunits with two N-linked oligosaccharides. Appearance of this glycoprotein on NK cells is IL-2 dependent and closely parallels IL-2-induced cytotoxicity against NK-resistant solid tumor cell targets.