Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression.
Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression.
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发表时间：
2006-03
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通讯作者：
Z. Isa;G. Schneider;R. Zaharias;D. Seabold;C. Stanford
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作者：
Z. Isa;G. Schneider;R. Zaharias;D. Seabold;C. Stanford
本研究的目的是验证氟化物修饰的钛表面会促进成骨细胞分化的假设。成骨细胞生长在一个中等粗糙的蚀刻氟化物改性钛表面（细胞分化的改变）进行了比较，成骨细胞生长在同一表面喷砂与二氧化钛。然后比较了纳米级改变对细胞形状和随后分化的潜在作用。材料与方法将人胚胎腭间充质（HEPM）培养物在相应表面上孵育1、3和7天，然后通过实时聚合酶链反应（PCR）分析细胞增殖、碱性磷酸酶（ALP）特异性活性和骨相关基因（ALP、I型胶原、骨钙素、骨唾液蛋白[BSP] II、Cbfa1和osterix）的mRNA稳态表达。结果：不同表面并不改变ALP、I型胶原、osterix、骨钙素或BSP II的mRNA表达。然而，在1周时，氟化物修饰的钛表面上的Cbfa1表达显著更高（P <0.001）。该表面上的细胞数比用25微米颗粒喷砂的表面TiO 2上的细胞数低20%，但与用125微米颗粒喷砂的表面TiO 2上的细胞数没有显著差异。生长在所有钛表面上的细胞表达相似水平的ALP活性。结论：结果表明，氟化物改性的表面形貌，协同表面粗糙度，可能有一个更大的影响Cbfa 1（成骨的关键调节器）的表达水平比未改性的钛表面研究。
PURPOSE The objective of this study was to test the hypothesis that fluoride-modified titanium surfaces would enhance osteoblast differentiation. Osteoblast growth on a moderately rough etched fluoride-modified titanium surface (alteration in cellular differentiation) was compared to osteoblast growth on the same surface grit-blasted with titanium dioxide. The potential role of nanometer-level alterations on cell shape and subsequent differentiation was then compared. MATERIALS AND METHODS Human embryonic palatal mesenchymal (HEPM) cultures were incubated on the respective surfaces for 1, 3, and 7 days, followed by analysis for cell proliferation, alkaline phosphatase (ALP) -specific activity, and mRNA steady-state expression for bone-related genes (ALP, type I collagen, osteocalcin, bone sialoprotein [BSP] II, Cbfa1, and osterix) by real-time polymerase chain reaction (PCR). RESULTS The different surfaces did not alter the mRNA expression for ALP, type I collagen, osterix, osteocalcin, or BSP II. However, Cbfa1 expression on the fluoride-modified titanium surface was significantly higher (P < .001) at 1 week. The number of cells on this surface was 20% lower than the number of cells on the surface TiO2-blasted with 25-microm particles but not significantly different from the number of cells on the surface TiO2-blasted with 125-microm particles. Cells grown on all the titanium surfaces expressed similar levels of ALP activity. CONCLUSIONS The results indicated that a fluoride-modified surface topography, in synergy with surface roughness, may have a greater influence on the level of expression of Cbfa1 (a key regulator for osteogenesis) than the unmodified titanium surfaces studied.