ANTIGENIC AND GENETIC-CHARACTERIZATION OF 3 INFLUENZA-C STRAINS ISOLATED IN THE KINKI DISTRICT OF JAPAN IN 1982-1983
ANTIGENIC AND GENETIC-CHARACTERIZATION OF 3 INFLUENZA-C STRAINS ISOLATED IN THE KINKI DISTRICT OF JAPAN IN 1982-1983
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DOI：
10.1016/0042-6822(89)90114-1
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发表时间：
1989-09-01
期刊：
影响因子：
3.7
通讯作者：
NAKAMURA, K
中科院分区：
文献类型：
--
作者：
ADACHI, K;KITAME, F;NAKAMURA, K
1982 年 2 月至 1983 年 12 月期间，从日本近畿地区的人类体内分离出三种丙型流感病毒株（C/Kyoto/41/82、C/Nara/82、C/Hyogo/1/83）。总 vRNA 的寡核苷酸图谱和抗 HE 单克隆抗体的抗原分析表明，它们彼此非常相似，但与之前在日本分离的任何株都不同。 1982年。在本研究中，确定了这三个菌株的血凝素酯酶（HE）基因的核苷酸序列，并与先前发表的菌株的核苷酸序列进行了比较。结果显示，近畿分离株与1980年美国分离的病毒（C/Mississippi/1/80）具有较高的核苷酸序列同源性（98.4-98.5%）。 C/Mississippi/1/80 Kinki 菌株的相似性也通过总 vRNA 的寡核苷酸图谱和使用一组 11 种抗 HE 单克隆抗体的抗原比较得到了证实。来自近畿和密西西比的分离株可以通过异质血清的血清学测试与先前分离的菌株区分开来，这表明它们可能在1982-1983年左右的日本具有流行病学优势。这些观察结果表明，与 C/Mississippi/1/80 密切相关的丙型流感病毒变种在 1982-1983 年在近畿发生了快速传播，大概是因为这种输入病毒的抗原性与以前流行的病毒有很大不同。
Three strains of influenza C virus (C/Kyoto/41/82, C/Nara/82, C/Hyogo/1/83) have been isolated from humans in the Kinki district of Japan between February 1982 and December 1983. Oligonucleotide mapping of total vRNA and antigenic analysis with anti-HE monoclonal antibodies have shown previously that they are closely similar to one another but dissimilar to any of the strains isolated in Japan before 1982. In the present study, the nucleotide sequences of the hemagglutinin-esterase (HE) genes of these three strains were determined and compared with those of the previously published strains. The results revealed that the Kinki isolates had a high nucleotide sequence homology (98.4-98.5%) with the virus isolated in 1980 in the United States (C/Mississippi/1/80). Similarity of the Kinki strains of C/Mississippi/1/80 was also confirmed by oligonucleotide mapping of total vRNA and antigenic comparison using a panel of 11 anti-HE monoclonal antibodies. The isolates from Kinki and Mississippi could be distinguished from the previously isolated strains in serological tests with heterogeneous sera, suggesting that they may have possessed epidemiological advantage in Japan around 1982-1983. These observations raise the possibility that the rapid spread of influenza C variant closely related to C/Mississippi/1/80 has occurred in Kinki in 1982-1983 presumably because this imported virus was largely different in antigenicity from the previously prevalent ones.