Gamma-oryzanol Prevents LPS-induced Brain Inflammation and Cognitive Impairment in Adult Mice
Gamma-oryzanol Prevents LPS-induced Brain Inflammation and Cognitive Impairment in Adult Mice
复制标题
DOI：
10.3390/nu11040728
复制
发表时间：
2019-04-01
期刊：
影响因子：
5.9
通讯作者：
Uberti, Daniela
中科院分区：
文献类型：
--
作者：
Mastinu, Andrea;Bonini, Sara Anna;Uberti, Daniela
背景：水稻（Oryza sativa L.）是一半以上人类的主要食物来源。大米富含植物化学物质和抗氧化剂，具有多种生物活性；在这些化合物中，谷维素的存在值得注意。本研究旨在探讨谷维素对神经炎症和认知改变小鼠模型认知表现的影响。方法：小鼠连续 21 天每天接受 100 mg/kg 谷维素 (ORY) 或载体，然后暴露于脂多糖 (LPS) 引发的炎症刺激物中。评估了一种新的物体识别测试以及海马抗氧化剂和神经炎症标记物的 mRNA 表达。结果：ORY 治疗能够改善神经炎症反应期间的认知表现。此外，ORY 和 ORY+LPS 小鼠海马中的 II 相抗氧化酶，如血红素加氧酶-1 (HO-1) 和 NADPH-脱氢酶-醌-1 (NQO1) 表达上调。最后，谷维素在 LPS 处理后通过下调炎症基因表现出强大的抗炎作用。结论：这些结果表明，长期摄入谷维素可以通过促进海马抗氧化和抗炎分子反应来恢复 LPS 引起的认知和记忆障碍。
Background: Rice (Oryza sativa L.) is the main food source for more than half of humankind. Rice is rich in phytochemicals and antioxidants with several biological activities; among these compounds, the presence of -oryzanol is noteworthy. The present study aims to explore the effects of -oryzanol on cognitive performance in a mouse model of neuroinflammation and cognitive alterations. Methods: Mice received 100 mg/kg -oryzanol (ORY) or vehicle once daily for 21 consecutive days and were then exposed to an inflammatory stimulus elicited by lipopolysaccharide (LPS). A novel object recognition test and mRNA expression of antioxidant and neuroinflammatory markers in the hippocampus were evaluated. Results: ORY treatment was able to improve cognitive performance during the neuroinflammatory response. Furthermore, phase II antioxidant enzymes such as heme oxygenase-1 (HO-1) and NADPH-dehydrogenase-quinone-1 (NQO1) were upregulated in the hippocampi of ORY and ORY+LPS mice. Lastly, -oryzanol showed a strong anti-inflammatory action by downregulating inflammatory genes after LPS treatment. Conclusion: These results suggest that chronic consumption of -oryzanol can revert the LPS-induced cognitive and memory impairments by promoting hippocampal antioxidant and anti-inflammatory molecular responses.