Molecular phenotype of airway side population cells
Molecular phenotype of airway side population cells
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DOI：
10.1152/ajplung.00149.2003
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发表时间：
2004-04-01
影响因子：
4.9
通讯作者：
Stripp, BR
中科院分区：
文献类型：
--
作者：
Giangreco, A;Shen, HM;Stripp, BR
肺上皮特异性干细胞已经定位于整个成人传导气道的离散微环境。这些细胞的特性包括耐污染性、多能分化和罕见增殖。本研究的目的是使用Hoechst 33342外排（其他组织中干细胞的一种特性）来纯化和进一步表征气道干细胞。Hoechst 33342流出的肺细胞通过流式细胞术被鉴定为维拉帕米敏感的侧群。肺侧群体细胞基于造血（CD 45阳性）或非造血（CD 45阴性）来源进一步细分。非造血侧群细胞富集干细胞抗原-1反应性，并表达气道和间充质谱系特异性的分子标志物。对气道衍生侧群细胞的分子表型的分析表明，它们与神经上皮体相关的变异Clara细胞相似。总之，这些数据表明，从肺分离的非造血侧群富集了先前鉴定的气道干细胞。
Lung epithelial-specific stem cells have been localized to discrete microenvironments throughout the adult conducting airway. Properties of these cells include pollutant resistance, multipotent differentiation, and infrequent proliferation. Goals of the present study were to use Hoechst 33342 efflux, a property of stem cells in other tissues, to purify and further characterize airway stem cells. Hoechst 33342 effluxing lung cells were identified as a verapamil-sensitive side population by flow cytometry. Lung side population cells were further subdivided on the basis of hematopoietic (CD45 positive) or nonhematopoietic (CD45 negative) origin. Nonhematopoietic side population cells were enriched for stem cell antigen-1 reactivity and expressed molecular markers specific to both airway and mesenchymal lineages. Analysis of the molecular phenotype of airway-derived side population cells indicates that they are similar to neuroepithelial body-associated variant Clara cells. Taken together, these data suggest that the nonhematopoietic side population isolated from lung is enriched for previously identified airway stem cells.