Heart- and skeletal muscle-specific antigens recognized by trichinellosis patient sera
Heart- and skeletal muscle-specific antigens recognized by trichinellosis patient sera
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DOI：
10.1111/j.1365-3024.2006.00889.x
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发表时间：
2006-09-01
影响因子：
2.2
通讯作者：
Bruschi, F.
中科院分区：
文献类型：
--
作者：
Pratesi, F.;Bongiorni, F.;Bruschi, F.
人类旋毛虫病可严重影响心脏，心脏受累可导致死亡。大鼠实验感染表明免疫病理过程可能参与其中。本文的目的是调查旋毛虫病患者血清中可能存在识别宿主组织，特别是心肌的抗体。对在波兹南地区（波兰）感染的晚期旋毛虫病患者的 19 份血清进行了免疫印迹检测，对正常大鼠或人的心室壁、脾脏、胎盘、肾脏和骨骼肌的提取物进行了检测。患者的血清识别出几种正常血清无法识别的抗原。在大鼠和人类心室壁上，高比例的血清（42%）与 68 kDa 的蛋白质发生反应（与正常血清相比，P < 0.05）。然而，在有或没有心脏受累的患者中，与该抗原的反应性没有显着差异。当对骨骼肌进行血清测试时，我们发现 47% 的血清与 27 kDa 的蛋白质发生反应，53% 的血清与 41 kDa 的蛋白质发生反应（与正常血清相比，两种蛋白质的 P < 0.05）。在肾脏、胎盘和脾提取物中未观察到针对 68 kDa 抗原以及针对 27 和 41 kDa 骨骼肌抗原的反应性。此外，与心脏和骨骼肌组织相比，在这些组织上观察到的条带非常少，因此表明旋毛虫病血清的反应性具有高组织特异性。总之，本研究在旋毛虫病患者血清中鉴定出了器官特异性自身抗体，但它们在心脏受累发病机制中的作用仍不清楚。
The heart can be seriously affected in human trichinellosis, and cardiac involvement can cause death. Experimental infections in rats have suggested the possible participation of immunopathological processes. The aim of the present paper was to investigate the possible presence in trichinellosis patient sera of antibodies recognizing host tissues and particularly the myocardium. Nineteen sera from late period trichinellosis patients, who acquired infection in the Poznan region (Poland), were tested by immunoblot on extracts from normal rat or human heart ventricle wall, spleen, placenta, kidney and skeletal muscle. Patients' sera recognized several antigens that were not recognized by normal sera. On rat and human heart ventricle wall, a high proportion of sera (42%) reacted with a protein of 68 kDa (P < 0.05 compared to normal sera). The reactivity with this antigen, however, was not significantly different in patients with or without cardiac involvement. When sera were tested on skeletal muscle we found that 47% reacted with a protein of 27 kDa and 53% reacted with a protein of 41 kDa (P < 0.05 for both proteins, compared with normal sera). The reactivity against the 68 kDa antigen and against the 27 and 41 kDa skeletal muscle antigens was not observed on kidney, placenta and spleen extracts. Moreover, very few bands were observed on these tissues as compared to heart and skeletal muscle tissues, thus suggesting a high tissue specificity of the reactivity of trichinellosis sera. In conclusion, this study identifies organ-specific autoantibodies in trichinellosis patient sera, their role in the pathogenesis of cardiac involvement being still unclear.