Serum Protein Profiling Reveals a Landscape of Inflammation and Immune Signaling in Early-stage COVID-19 Infection.
Serum Protein Profiling Reveals a Landscape of Inflammation and Immune Signaling in Early-stage COVID-19 Infection.
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血清蛋白分析揭示了早期 COVID-19 感染中的炎症和免疫信号传导情况
DOI：
10.1074/mcp.rp120.002128
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Li Y
中科院分区：
文献类型：
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作者：
Hou X;Zhang X;Wu X;Lu M;Wang D;Xu M;Wang H;Liang T;Dai J;Duan H;Xu Y;Yu X;Li Y
我们使用高密度抗体微阵列对早期新冠病毒（COVID - 19）患者的血清进行了深入的蛋白质组学分析。我们鉴定出大量差异表达的蛋白质（n = 132），这些蛋白质构成了与新冠病毒感染相关的炎症和免疫信号图谱。此外，我们还观察到中性粒细胞和淋巴细胞与CCL2和CXCL10介导的细胞因子信号通路之间存在显著相关性。这些信息对于理解新冠病毒的发病机制、生物标志物的识别以及最佳抗炎治疗的开发具有重要价值。
要点
对早期新冠病毒患者血清蛋白质组的深入分析。
与新冠病毒感染相关的炎症和免疫信号图谱。
CCL2和CXCL10介导的细胞因子信号通路可能分别与中性粒细胞和淋巴细胞相关。
2019冠状病毒病（COVID - 19）是一种具有高度传染性的感染疾病，威胁着全球人类的生命。血液中细胞因子的升高对于在新冠病毒患者病情严重程度转变过程中引发细胞因子风暴和免疫抑制至关重要。然而，在整个新冠病毒感染过程中，新冠病毒患者血清蛋白质的全面变化尚不清楚。在这项工作中，我们开发了一种高密度抗体微阵列，并对从早期新冠病毒（n = 15）和流感（n = 13）患者收集的血清样本进行了深入的蛋白质组学分析。我们鉴定出大量差异表达的蛋白质（n = 132），这些蛋白质参与了与新冠病毒感染相关的炎症和免疫信号图谱。此外，我们还确定了中性粒细胞和淋巴细胞与CCL2和CXCL10介导的细胞因子信号通路之间的显著相关性。这些信息对于理解新冠病毒的发病机制、生物标志物的识别以及最佳抗炎治疗的开发具有重要价值。
We performed an in-depth proteomics analysis of serum of early COVID-19 patients using a high-density antibody microarray. We identified a large set of differentially expressed proteins (n = 132) that constitute a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways were observed. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy. Graphical Abstract Highlights In-depth profiling of the serum proteome in early-stage COVID-19 patients. A landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. CCL2 and CXCL10 medicated cytokine signaling pathways may correlate with neutrophil and lymphocyte respectively. Coronavirus disease 2019 (COVID-19) is a highly contagious infection and threating the human lives in the world. The elevation of cytokines in blood is crucial to induce cytokine storm and immunosuppression in the transition of severity in COVID-19 patients. However, the comprehensive changes of serum proteins in COVID-19 patients throughout the SARS-CoV-2 infection is unknown. In this work, we developed a high-density antibody microarray and performed an in-depth proteomics analysis of serum samples collected from early COVID-19 (n = 15) and influenza (n = 13) patients. We identified a large set of differentially expressed proteins (n = 132) that participate in a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways was identified. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy.