CD8 binding to MHC class I molecules is influenced by maturation and T cell glycosylation
CD8 binding to MHC class I molecules is influenced by maturation and T cell glycosylation
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DOI：
10.1016/s1074-7613(01)00252-7
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发表时间：
2001-12-01
期刊：
影响因子：
32.4
通讯作者：
Jameson, SC
中科院分区：
文献类型：
--
作者：
Daniels, MA;Levine, L;Jameson, SC
CD8既可作为I类MHC分子的粘附分子，又可作为TCR的辅助受体，用于T细胞活化。在这里，我们研究了cd8介导的与非同源肽/MHC配体（即不与TCR结合的配体）结合的发育调节。我们发现CD8结合可溶性I类MHC四聚体和在剪切流动条件下介导T细胞粘附的能力随着双阳性胸腺细胞成熟为CD8(+) T细胞而减弱。此外，我们提供的证据表明，这种CD8结合的减少是由于T细胞成熟时唾液化的增加。这些数据表明CD8与I类MHC相互作用的能力不是固定的，而是通过T细胞的糖基化状态进行发育调节的。
CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8(+) T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.