Inability to elicit rapid cytocidal effects on L1210 cells derived from porphyrin-injected mice following in vitro photoirradiation.
Inability to elicit rapid cytocidal effects on L1210 cells derived from porphyrin-injected mice following in vitro photoirradiation.
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体外光照射后，无法对来自注射卟啉的小鼠的 L1210 细胞产生快速杀细胞作用。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
N. Datta
中科院分区：
文献类型：
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作者：
D. Musser;N. Datta
利用一系列肿瘤定位卟啉和L1210肿瘤系统，研究了肿瘤细胞在卟啉定位和光化学治疗反应中的作用。在体内光照射DBA/2 Ha小鼠L1210实体瘤和先前给予注射meso-四-（4-sulfonatophenyl）-卟啉，meso-四-（4-carboxyphenyl）-卟啉，或血卟啉衍生物（HPD）表明，所有三种化学品引起的光动力学反应，导致暴露的组织坏死。使用温和的机械手段和生理条件从给予卟啉注射的小鼠中分离肿瘤细胞，然后在建立优化快速杀细胞作用的条件下对细胞进行体外光照射，结果没有明显的细胞死亡。在使用来自给予Hpd注射的小鼠的脾细胞时注意到类似的情况，脾细胞可能含有大量的卟啉。荧光显微镜和化学提取和定量的卟啉在细胞中表明，无法引起快速的细胞杀伤作用后，在体外光照射导致的不存在或存在的只有非常少量的卟啉。这些结果表明，在这个特定的肿瘤系统中，肿瘤细胞本身在卟啉定位中仅起次要作用，因此，不能在光照射后被轻易杀死，这表明，仅由于细胞内含有的卟啉，在体内光照射过程中，大多数实质细胞中可能不会发生快速的杀细胞作用。
The role of the neoplastic cell in both porphyrin localization and the photochemotherapeutic response was investigated with the use of a series of tumor-localizing porphyrins and the L1210 tumor system. In vivo photoirradiation of DBA/2Ha mice bearing L1210 solid tumors and previously given injections of meso-tetra-(4-sulfonatophenyl)-porphine, meso-tetra-(4-carboxyphenyl)-porphine, or hematoporphyrin derivative (Hpd) indicated that all three chemicals elicited a photodynamic response resulting in necrosis of exposed tissue. Isolation of tumor cells from mice given injections of porphyrin with the use of mild mechanical means and physiologic conditions followed by in vitro photoirradiation of the cells under conditions established to optimize rapid cytocidal effects resulted in no appreciable cell death. A similar situation was noted with the use of spleen cells from mice given injections of Hpd, the spleen cells presumably containing substantial amounts of porphyrin. Both fluorescence microscopy and chemical extraction and quantitation of the porphyrins in the cells indicated that the inability to elicit a rapid cytocidal effect upon in vitro photoirradiation resulted from either the absence of or the presence of only very small amounts of porphyrin. These results indicate that in this particular tumor system the neoplastic cell per se plays only a minor role in porphyrin localization and, as a consequence, cannot be readily killed upon photoirradiation, suggesting that rapid cytocidal effects, due solely to porphyrin contained within the cell, probably do not occur among the majority of parenchymal cells during in vivo photoirradiation.