(Na+ +K+)-ATPase activity in kidney basolateral membranes of non insulin dependent diabetic rats.
(Na+ +K+)-ATPase activity in kidney basolateral membranes of non insulin dependent diabetic rats.
复制标题
非胰岛素依赖型糖尿病大鼠肾基底外侧膜的 (Na K )-ATP 酶活性。
DOI：
10.1016/s0006-291x(86)80321-7
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Gavin3rd,JR
中科院分区：
文献类型：
--
作者：
Levy,J;Avioli,LV;Roberts,ML;Gavin3rd,JR
在大鼠动物模型中，胰岛素抵抗的II型糖尿病（NIDD）导致肾基底外侧膜（BLM）制备物中基底膜和胰岛素刺激的膜（Ca 2 ++ Mg 2+）-ATP酶活性的显著变化。我们发现，NIDD在这些动物中并没有导致类似的膜（Na++K+）-ATP酶活性的变化。糖尿病组和对照组动物的基础酶活性相同。在体糖尿病动物经胰岛素治疗后，BLM（Na++K+）-ATP酶活性升高，而限食18小时后酶活性降低。在任何动物组的离体膜中，胰岛素对（Na++K+）-ATP酶活性均无直接影响。因此，改变胰岛素敏感性和葡萄糖稳态的生理扰动伴随着（Na++K+）-ATP酶活性水平的改变。较低水平的这种膜酶活性似乎与最佳胰岛素作用有关。
Insulin resistant, Type II diabetes mellitus (NIDD) in a rat animal model results in profound changes in basal and insulin-stimulated membrane (Ca2++Mg2+)-ATPase activity in kidney basolateral membrane (BLM) preparations. We find that NIDD in these animals does not result in similar changes in membrane (Na++K+)-ATPase activity. Basal enzyme activity was the same in diabetic and control animals. Insulin treatment of diabetic animalsin vivoresulted in hyperinsulinemia and increased BLM (Na++K+)-ATPase, while food restriction for 18 hr resulted in lowered enzyme activity. There was no direct effect of insulin on (Na++K+)-ATPase activity in isolated membranes from any of the animal groups. Thus, physiologic perturbations which alter insulin sensitivity and glucose homeostasis are accompanied by altered levels of (Na++K+)-ATPase activity. Lower levels of this membrane enzyme activity appear to be associated with optimal insulin action.