Gfi1-Cre knock-in mouse line: A tool for inner ear hair cell-specific gene deletion.
Gfi1-Cre knock-in mouse line: A tool for inner ear hair cell-specific gene deletion.
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作者：
Yang, Hua;Gan, Jean;Xie, Xiaoling;Deng, Min;Feng, Liang;Chen, Xiaowei;Gao, Zhiqiang;Gan, Lin
gfi 1编码一种锌指转录因子，对造血和内耳的发育和维持至关重要。在小鼠内耳中，Gfi 1表达仅限于发育期间和成年期的毛细胞。为了构建内耳毛细胞特异性基因缺失的遗传工具，我们通过将Cre编码序列敲入Gfi 1位点并使内源性Gfi 1失活来产生Gfi 1-Cre小鼠系。通过条件R26 R-lacZ报告基因的表达揭示了Gfi 1-Cre重组酶介导的重组在发育中内耳中的特异性和效率。LacZ在Gfi 1Cre/+内耳中的表达开始于前庭中的E13.5和耳蜗中的E15.5，与毛细胞的产生一致。在整个内耳发育过程中，lacZ的表达仅在毛细胞中检测到。因此，Gfi 1-Cre基因敲入小鼠系提供了一个有用的工具，基因操作，特别是在内耳毛细胞。
Gfi1encodes a zinc-finger transcription factor essential for the development and maintenance of haematopoiesis and the inner ear. In mouse inner ear, Gfi1 expression is confined to hair cells during development and in adulthood. To construct a genetic tool for inner ear hair cell-specific gene deletion, we generated a Gfi1-Cre mouse line by knocking-in Cre coding sequences into the Gfi1 locus and inactivating the endogenous Gfi1. The specificity and efficiency of Gfi1-Cre recombinase-mediated recombination in the developing inner ear was revealed through the expression of the conditional R26R-lacZ reporter gene. The onset of lacZ expression in the Gfi1Cre/+ inner ear was first detected at E13.5 in the vestibule and at E15.5 in the cochlea, coinciding with the generation of hair cells. Throughout inner ear development, lacZ expression was detected only in hair cells. Thus, Gfi1-Cre knock-in mouse line provides a useful tool for gene manipulations specifically in inner ear hair cells.
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影响因子：
7.7
作者：
Fiolka, K;Hertzano, R;Möröy, T
通讯作者：
Möröy, T
影响因子：
2.7
作者：
Gan, L;Wang, SW;Klein, WH
通讯作者：
Klein, WH
影响因子：
5.3
作者：
GILKS, CB;BEAR, SE;TSICHLIS, PN
通讯作者：
TSICHLIS, PN
影响因子：
10.5
作者：
Shroyer, NF;Wallis, D;Zoghbi, HY
通讯作者：
Zoghbi, HY
DOI：
10.1523/jneurosci.5237-08.2009
发表时间：
2009-04-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Ding Q;Chen H;Xie X;Libby RT;Tian N;Gan L
通讯作者：
Gan L