Evaluating cell wall structure and composition of developing cotton fibers using Fourier transform infrared spectroscopy and thermogravimetric analysis
Evaluating cell wall structure and composition of developing cotton fibers using Fourier transform infrared spectroscopy and thermogravimetric analysis
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DOI：
10.1002/app.27100
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发表时间：
2008-01-05
影响因子：
3
通讯作者：
Wells, Leslie W.
中科院分区：
文献类型：
--
作者：
Abidi, Noureddine;Hequet, Eric;Wells, Leslie W.
使用通用衰减全反射傅里叶变换红外（UATR-FTIR）光谱和热重分析（TGA）来研究棉花（Gossypium hirsutum L.）纤维的结构变化作为发育规划的函数。从 10、14、17 和 20 dpa（花后天）纤维的 FTIR 光谱中可以明显看出非纤维素化合物（蜡、蛋白质、半纤维素、果胶物质、氨基酸等）的存在。与非纤维素化合物相对应的振动在 36 dpa 时消失。此外，独立的 TGA 分析支持 FTIR 获得的结果，表明从初生细胞壁合成到次生细胞壁合成的转变发生在 20 dpa 或左右。这项研究首次报告了使用 UATR-FTIR 和 TGA 来阐明棉纤维发育过程中的结构变化。 (C) 2007 年 Wiley 期刊公司。
Universal attenuated total reflectance Fourier transform infrared (UATR-FTIR) spectroscopy and thermogravimetric analysis (TGA) were used to investigate the structural changes of cotton (Gossypium hirsutum L.) fibers as a function of developmental programming. The presence of noncellulosic compounds (wax, protein, hemicelluloses, pectic substances, amino acids, etc.) was evident from FTIR spectra of fibers at 10, 14, 17, and 20 dpa (day postanthesis). The vibration corresponding to the noncellulosic compounds disappeared at 36 dpa. Furthermore, independent TGA analysis supported the results obtained with FTIR, showing that the transition from primary cell wall synthesis to secondary cell wall synthesis occurs at or around 20 dpa. This study is the first to report on the use of the UATR-FTIR and TGA to elucidate structural changes during cotton fiber development. (C) 2007 Wiley Periodicals, Inc.