Molecular phylogeny of Flaveria as deduced from the analysis of nucleotide sequences encoding the H-protein of the glycine cleavage system
Molecular phylogeny of Flaveria as deduced from the analysis of nucleotide sequences encoding the H-protein of the glycine cleavage system
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DOI：
10.1111/j.1365-3040.1996.tb00209.x
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发表时间：
1996-09-01
影响因子：
7.3
通讯作者：
Bauwe, H
中科院分区：
文献类型：
--
作者：
Kopriva, S;Chu, CC;Bauwe, H
序列比较表明，核苷酸序列的H-蛋白，甘氨酸裂解系统的一个组成部分，只有适度保守，可用作分子掩蔽物的属内系统发育研究。我们已经分析了各自的cDNA序列从12种黄，和更有限的一组gdcsH上游区域。这些数据进行了讨论相对于一个系统发育重建的黄，一个小属，其中包括不同的光合类型，即C-3，C-3-C-4，C-4样和C-4的物种。我们的分析基本上支持了一个早期的假设，基于形态和生态地理数据，黄菊的进化（鲍威尔1978）。这种密切的协议表明H-蛋白核苷酸序列在低分类水平的有用性。我们的分析独立地证实，C-4光合作用在不同的黄菊谱系中进化了两次。最值得注意的是，黄菊属的C-4分类群似乎是相对于C-3-C-4中间分类群衍生的，即它们可能有共同的直接前身。这是C-3-C-4中间光合作用在植物遗传学上处于中间位置的第一个直接证据。我们的数据也证实了黄菊属植物的钙光合作用的古老性，但表明F。在我们的实验中使用的pringlei，虽然明显的C，光合代谢，可能起源于杂交与一个更先进的分类。
Sequence comparisons have shown that nucleotide sequences of the H-protein, a component of the glycine cleavage system, are only moderately conserved and can be used as molecular maskers for intrageneric phylogenetic studies. We have analysed the respective cDNA sequences from 12 species of Flaveria, and a more limited set of gdcsH upstream regions. These data are discussed with respect to a phylogenetic reconstruction of Flaveria, a small genus which includes species of different photosynthetic types, namely C-3, C-3-C-4, C-4-like and C-4. Our analysis essentially supports an earlier hypothesis, based an morphological and eco-geographical data, of the evolution of Flaveria (Powell 1978). This close agreement shows the usefulness of H-protein nucleotide sequences at a low taxonomic level. Our analysis independently confirms that C-4 photosynthesis has evolved two times in different lineages of Flaveria. Most remarkably, the C-4 taxa of Flaveria appear as derived relative to the C-3-C-4 intermediate taxa, i.e. they probably have common direct predecessors. This is the first direct evidence for a phylogenetically intermediate position of C-3-C-4 intermediate photosynthesis. Our data also confirm the antiquity of Ca photosynthesis in Flaveria but suggest that the collection of F. pringlei used in our experiments, although clearly of C, photosynthetic metabolism, possibly originated from hybridization with a more advanced taxon.