An evaluation of the performance of ten commercial luminometers.
An evaluation of the performance of ten commercial luminometers.
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对十种商用发光计的性能评估。
DOI：
10.1002/bio.1170030307
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
B. Mccarthy
中科院分区：
文献类型：
--
作者：
P. Jago;W. J. Simpson;S. Denyer;A. Evans;M. Griffiths;J. Hammond;T. P. Ingram;R. Lacey;N. W. Macey;B. Mccarthy
对萤火虫荧光素酶法定量测定三磷酸腺苷（ATP）的10种仪器的相对性能进行了评估。评价的仪器是阿默舍姆Amerlite分析仪、Dynatech管光度计、Dynatech多板光度计、Dynatech照相光度计、汉密尔顿Lumicon、LKB 1250光度计、LKB 1251光度计、Lumac Biocounter M2010 A、Turner 20 TD光度计和CLEAR SpeedTech 2000的原型版本。在最敏感的（Lumac，Turner）和最不敏感的（Dynatech管）之间发现了800倍的灵敏度差异。Dynatech相机光度计的工作原理与其他仪器完全不同，其灵敏度比最好的光电倍增管仪器低5000倍。无论分析ATP水溶液还是三氯乙酸细菌提取物，仪器的相对灵敏度都保持不变。对960个ATP生物发光测定的分析表明，从这些测量中获得的数据呈正态分布。
An assessment has been carried out of the relative performance of ten instruments for quantification of adenosine triphosphate (ATP) by the firefly luciferase assay. The instruments evaluated were Amersham Amerlite Analyser, Dynatech Tube Luminometer, Dynatech Multiplate Luminometer, Dynatech Camera Luminometer, Hamilton Lumicon, LKB 1250 Luminometer, LKB 1251 Luminometer, Lumac Biocounter M2010A, Turner 20 TD Luminometer and a prototype version of the CLEAR SpeedTech 2000. An 800-fold difference in sensitivity was found between the most sensitive (Lumac, Turner) and the least sensitive (Dynatech Tube) of the conventional instruments. The Dynatech Camera Luminometer which worked on a completely different principle to the other instruments was about 5000 times less sensitive than the best of the photomultiplier tube instruments. The relative sensitivity of the instruments was maintained regardless of whether solutions of ATP in water or trichloroacetic acid extracts of bacteria were analysed. An analysis of 960 ATP bioluminescence assays showed that data obtained from such measurements are normally distributed.