Fusarium oxysporum f. sp. lycopersici C2H2 transcription factor FolCzf1 is required for conidiation, fusaric acid production, and early host infection
Fusarium oxysporum f. sp. lycopersici C2H2 transcription factor FolCzf1 is required for conidiation, fusaric acid production, and early host infection
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DOI：
10.1007/s00294-019-00931-9
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发表时间：
2019-01
期刊：
影响因子：
2.5
通讯作者：
Yingzi Yun;Xin Zhou;Shuai Yang;Y. Wen;Haixia You;Yuru Zheng;Justice Norvienyeku;W. Shim;Zonghua Wang
中科院分区：
文献类型：
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作者：
Yingzi Yun;Xin Zhou;Shuai Yang;Y. Wen;Haixia You;Yuru Zheng;Justice Norvienyeku;W. Shim;Zonghua Wang
番茄枯萎病菌是一种土传无性真菌，是番茄枯萎病的病原。病原菌的侵染过程包括根的识别、黏附、穿透、根皮层的定植和木质部导管内的菌丝增殖，这些过程都受毒力相关转录因子(TF)的调控。在本研究中，我们鉴定了一个基因，命名为FolCZF1，它编码一个C2H2 Tf in Fol。已知的FolChf1的同系物也影响致病性inf。小麦和水稻上分别有禾本科和稻瘟病菌。我们了解到FolCZF1转录水平在分生孢子和寄主早期感染阶段上调，这使得我们推测Folczf1与寄主早期感染Fol有关。FolCZF1缺失突变体(ΔFolCZF1)在生长速度、分生孢子、分生孢子形态等方面存在缺陷，对番茄根部的致病力完全丧失。进一步的显微镜观察表明，ΔFolCZF1能穿透根部，但初侵染的菌丝不能在寄主组织内扩展其定植，这表明Folzf1 Tf在早期侵染中起着重要作用。镰刀菌产生的次生代谢物镰刀菌酸被认为是许多作物病害的致病因子。我们发现Folzzf1通过调节Fusaric酸生物合成基因的表达而在Fusaric酸的生产中起着关键作用。综上所述，FOL中的分生孢子、次生代谢和早期寄主感染都需要Folzzf1的同源基因，我们认为在其他植物病原丝状真菌的早期寄生生长中也需要Folzzf1的同源基因。
The soil-borne, asexual fungusFusarium oxysporumf.sp.lycopersici(Fol) is a causal agent of tomato wilt disease. The infection process ofFolcomprises root recognition, adhesion, penetration, colonization of the root cortex and hyphal proliferation within the xylem vessels, which are under the regulation of virulence-involved transcription factors (TFs). In this study, we identified a gene, designatedFolCZF1, which encodes a C2H2TF inFol. The homologs of FolCzf1 are also known to affect pathogenicity inF. graminearumandMagnaporthe oryzaeon wheat and rice, respectively. We learned thatFolCZF1transcript level is upregulated in conidia and early host infection stage, which led us to hypothesize that FolCzf1 is associated with early host infection inFol. TheFolCZF1deletion mutant (ΔFolCZF1) exhibited defects in growth rate, conidiation, conidia morphology and a complete loss of virulence on tomato root. Further microscopic observation showed that ΔFolCZF1can penetrate the root but the primary infection hypha cannot extend its colonization inside the host tissue, suggesting that FolCzf1 TF plays an important role in early infection. Fusaric acid, a secondary metabolite produced byFusariumspecies, is suggested as a virulence factor in many crop diseases. We found that FolCzf1 plays a critical role in fusaric acid production by regulating the expression of fusaric acid biosynthesis genes. In summary, FolCzf1 is required for conidiation, secondary metabolism, and early host infection inFol, and we propose that homologs of FolCzf1 are required for early parasitic growth in other plant pathogenic filamentous fungi.