PROTEINS REQUIRED FOR ULTRAVIOLET-LIGHT AND CHEMICAL MUTAGENESIS - IDENTIFICATION OF THE PRODUCTS OF THE UMUC LOCUS OF ESCHERICHIA-COLI
PROTEINS REQUIRED FOR ULTRAVIOLET-LIGHT AND CHEMICAL MUTAGENESIS - IDENTIFICATION OF THE PRODUCTS OF THE UMUC LOCUS OF ESCHERICHIA-COLI
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DOI：
10.1016/0022-2836(83)90074-8
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
WALKER, GC
中科院分区：
文献类型：
--
作者：
ELLEDGE, SJ;WALKER, GC
umuC是大肠杆菌中导致紫外线和化学诱变的易出错修复过程中表现出最大特异性的遗传位点。通过生成umuC DNA特异性探针克隆了umuC基因座。通过亚克隆和Tn1000诱变相结合，获得了2.2倍的区域。鉴定出103个包含补充umuC突变所需信息的碱基。这一区域的DNA编码两个多肽，分子量分别为16000和45000。这些蛋白质的基因被组织在一个被lexA蛋白抑制的操纵子中。对先前分离的umuC突变的互补发现，这些蛋白对应于2个互补基团，umuC编码45000 MW的蛋白，umuD编码16000 MW的蛋白。这两种蛋白质都是大肠杆菌易出错修复所必需的。
umuC is the genetic locus showing the greatest specificity for the error-prone repair process that is responsible for UV and chemical mutagenesis in E. coli. The umuC locus was cloned by generating a probe specific to umuC DNA. Through a combination of subcloning and Tn1000 mutagenesis, a region of 2.2 .times. 103 bases which contains the information necessary to complement umuC mutations was identified. This region of DNA codes for 2 polypeptides with MW of 16,000 and 45,000. The genes for these proteins are organized in an operon that is repressed by the lexA protein. Complementation of previously isolated umuC mutations revealed that these proteins correspond to 2 complementation groups, umuC, which codes for the 45,000 MW protein and umuD, which codes for the 16,000 MW protein. Both proteins are essential for error-prone repair in E. coli.