Zebrafish and fly Nkx6 proteins have similar CNS expression patterns and regulate motoneuron formation
Zebrafish and fly Nkx6 proteins have similar CNS expression patterns and regulate motoneuron formation
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DOI：
10.1242/dev.01397
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发表时间：
2004-11-01
期刊：
影响因子：
4.6
通讯作者：
Eisen, JS
中科院分区：
文献类型：
--
作者：
Cheesman, SE;Layden, MJ;Eisen, JS
属于Nkx，GSH和Msx家族的基因在昆虫和脊椎动物中枢神经系统（CNS）的类似背腹空间域中表达，表明双侧祖先在CNS发育期间使用这种遗传程序。我们已经调查了这些相似的表达模式的意义，通过测试是否Nkx6蛋白表达在腹侧中枢神经系统的斑马鱼和苍蝇有相似的功能。在斑马鱼中，Nkx6.1在早期出生的初级和晚期出生的次级运动神经元中表达。在Nkx6.1的情况下，有较少的次级运动神经元和多余的腹侧中间神经元，表明Nkx6.1促进运动神经元和抑制中间神经元的形成。鱼类或苍蝇Nkx6的过表达足以在斑马鱼和苍蝇中产生多余的运动神经元。这些结果表明Nkx6蛋白的一个祖先功能是促进运动神经元发育。
Genes belonging to the Nkx, Gsh and Msx families are expressed in similar dorsovental spatial domains of the insect and vertebrate central nervous system (CNS), suggesting the bilaterian ancestor used this genetic program during CNS development. We have investigated the significance of these similar expression patterns by testing whether Nkx6 proteins expressed in ventral CNS of zebrafish and flies have similar functions. In zebrafish, Nkx6.1 is expressed in early-born primary and later-born secondary motoneurons. In the absence of Nkx6.1, there are fewer secondary motoneurons and supernumerary ventral interneurons, suggesting Nkx6.1 promotes motoneuron and suppresses interneuron formation. Overexpression of fish or fly Nkx6 is sufficient to generate supernumerary motoneurons in both zebrafish and flies. These results suggest that one ancestral function of Nkx6 proteins was to promote motoneuron development.