Noninvasive biosensor for cathepsin L in the stratum corneum.
Noninvasive biosensor for cathepsin L in the stratum corneum.
复制标题
用于角质层组织蛋白酶 L 的无创生物传感器。
DOI：
10.1111/j.1600-0846.2011.00565.x
复制
发表时间：
2012
期刊：
影响因子：
2.2
通讯作者：
Shimizu T.
中科院分区：
文献类型：
--
作者：
Yamaguchi M;Date A;Sasaki M;Makino T;Shimizu T.
目的提出一种现场检测角质层组织蛋白酶L活性的生物传感器，该传感器由一次性试纸和台式阅读器(260×150×290×290 mm~3，1.9 mm~3)组成，结合电荷耦合器件图像传感器单元测量试纸的反射率。提出了一种新的用于角质层CATL分析的免疫层析试纸。为了实现试条的建立，选择了胶体金技术作为CATL的分子识别方法。采用胶带剥离法无创采集人体皮肤样品。结果根据优化的检测条件，对该生物传感器的灵敏度进行了评价。从滴下样品开始，需要10分钟才能在测试条上出现测试线。光密度与CATL成正比。生物分析验证表明，在生物传感器的检测下限(172.2μU/mL)内，其准确度(R2=100.94)和精密度(CV=115%)接近于更精密的实验室分析仪。此外，截短的采样-报告周期(<；15分钟)允许快速报告CATL水平。结论该生物传感器具有无创、使用方便的特点，有望成为角质层CATL半定量分析的新工具。
PurposeThe objective is to propose an on‐site testing biosensor of cathepsin L (CatL) activity in the stratum corneum, which can be used for the evaluation of skin conditions noninvasively and easily.MethodsThe biosensor comprises of a disposable test strip and a desktop‐sized reader (260 × 150 × 290 mm3, 1.9 kg), incorporating a charge‐coupled device image sensor (CCD) unit to measure the reflectance of the test strip. A novel immuno‐chromatographic test strip was proposed for CatL analysis in the stratum corneum. In order to realize the test strip, a colloidal gold technique was selected as the molecular recognition method for the CatL. A human skin sample was collected noninvasively by adhesive tape stripping.ResultsBased on optimal assay conditions, the sensitivity of the biosensor was evaluated. It required 10 min from a sample dropping to appear the test line on the test strip. The optical density was proportion to the CatL. Bioanalytical validation indicated that, within the biosensor's detection limit (172.2 μU/mL), its accuracy (R2= 0.94), and precision (CV = 15%) approach more elaborate laboratory‐based analyzers. In addition, the truncated sampling‐reporting cycle (<15 min) allows speedy reporting of CatL levels.ConclusionIt was indicated that this noninvasive and easy‐to use biosensor might be a novel tool for the semi‐quantitative analysis of CatL in the stratum corneum.