A Mycobacterium avium subsp paratuberculosis LuxR regulates cell envelope and virulence
A Mycobacterium avium subsp paratuberculosis LuxR regulates cell envelope and virulence
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DOI：
10.1177/1753425909339811
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发表时间：
2010-08-01
期刊：
影响因子：
3.2
通讯作者：
Bermudez, Luiz E.
中科院分区：
文献类型：
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作者：
Alonso-Hearn, Marta;Eckstein, Torsten M.;Bermudez, Luiz E.
鸟分枝杆菌副结核杆菌通过调节影响其包膜组成的基因来适应环境。在牛奶中生长的细菌（体内条件）在细胞壁相关脂质和参与脂质代谢（FadE 8，FadE 6和MAP 1420）和宿主细胞侵袭（MAP 1203，LprL）的基因表达方面存在差异。一个不同的脂质分布也观察到在1小时的感染后的细胞内细菌的包膜。胞内细菌显示LuxR调节剂的上调，其通过上调FadE 8、MAP 1420、MAP 1203和LprL以及下调pks 12、mmpL 2和MAP 2594来控制包膜的组成。具有脂质缺陷包膜表型的LuxR过表达菌株比野生型细菌更有效地感染上皮细胞;然而，它并不比野生型菌株对杀菌蛋白的作用更具抗性。在这里，我们表明，LuxR调节毒力决定因素，并参与分枝杆菌适应宿主。
Mycobacterium avium subsp. paratuberculosis adapts to the environment via the regulation of genes affecting its envelope's composition. Bacteria grown in milk (in vivo conditions) presented differences in the cell wall-associated lipids and in the expression of genes involved in lipid metabolism (FadE8, FadE6 and MAP1420) and host cell invasion (MAP1203, LprL). A different lipid profile was also observed in the envelope of intracellular bacteria after 1 h of infection. Intracellular bacteria showed up-regulation of a LuxR regulator which controls the envelope's composition by up-regulation of FadE8, MAP1420, MAP1203 and LprL and by down-regulation of pks12, mmpL2 and MAP2594. A LuxR-overexpressing strain with a lipid-deficient envelope phenotype, infected epithelial cells more efficiently than the wild-type bacteria; however, it was not more resistant than the wild-type strain to the action of bactericidal proteins. Here we show that LuxR regulates virulence determinants and is involved in mycobacteria adaptation to the host.