Role of Surface Coverage of Sessile Probiotics in Their Interplay with Pathogen Bacteria Investigated by Digital Holographic Microscopy
Role of Surface Coverage of Sessile Probiotics in Their Interplay with Pathogen Bacteria Investigated by Digital Holographic Microscopy
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DOI：
10.1021/acs.langmuir.3c02436
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发表时间：
2023-11-16
期刊：
影响因子：
3.9
通讯作者：
Zhang，Guangzhao
中科院分区：
文献类型：
--
作者：
He，Xintong;Zhang，Weixiong;Zhang，Guangzhao
益生菌的粘附在胃肠道中起着重要的作用。了解益生菌覆盖范围对肠道病原体的影响对于设计有效的益生菌疗法至关重要。在本研究中，我们利用家用数字全息显微镜研究了肠道致病菌坂崎肠杆菌（enterobacter sakazaki， ES）在涂有益生菌─鼠李糖乳杆菌（Lactobacillus rhamnosusGG， LGG）表面附近的适应行为与表面覆盖率（CRLGG）的关系。结果表明，即使crlgg值低至0.05%，ES细胞也能在4.2 μm范围内自适应感知LGG。crlgg对胚胎干细胞生长的抑制作用略有不同，但胚胎干细胞近地表加速和积累对crlgg的依赖性较大。在crlgg从0.05 ~ 24.6%的范围内，主动游泳的ES百分比、运动偏倚、加速度和相互作用持续时间均不随crlgg呈线性变化。相反，它们都表现出13.4%的极端atCRLGG，对应于由LGG释放的扩散刺激（有机酸、细菌素等）诱导的ES细胞趋化行为，模拟显示出atCRLGG= 13.4%的极端浓度梯度。我们的研究清楚地表明，无根益生菌的表面覆盖深度影响它们与病原菌的相互作用，在设计益生菌疗法时应考虑到这一点。
The adhesion of probiotics plays an important role in the gastrointestinal tract. Understanding the effect of the coverage of colonized probiotics on enteric pathogens is critical for the design of effective probiotic therapies. In the present work, we have investigated the adaptive behaviors of the intestinal pathogenic bacteriaEnterobacter sakazakii(ES) near the surfaces coated with a probiotic─Lactobacillus rhamnosusGG (LGG) as a function of surface coverage ratio (CRLGG) by using a home-setup digital holographic microscopy. It shows that ES cells can adaptively sense LGG within a distance of 4.2 μm, even atCRLGGvalues as low as 0.05%. The growth inhibition of ES cells slightly varies withCRLGG, but the near-surface acceleration and accumulation of ES cells have much dependence onCRLGG. AsCRLGGincreases from 0.05 to 24.6%, the percentage of actively swimming ES, the motion bias, the acceleration, and the interplay duration do not linearly vary withCRLGG. Instead, each of them shows an extreme atCRLGGof 13.4%, corresponding to the chemotaxis behaviors of ES cells induced by diffusing stimuli (organic acids, bacteriocins,etc.) released from LGG, which showed an extreme concentration gradient atCRLGG= 13.4% by simulations. Our study clearly demonstrates that surface coverage of sessile probiotics profoundly influences their interplay with pathogen bacteria, which should be taken into account in designing probiotic therapies.