Development of the mouse suprachiasmatic nucleus: Determination of time of cell origin and spatial arrangements within the nucleus
Development of the mouse suprachiasmatic nucleus: Determination of time of cell origin and spatial arrangements within the nucleus
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DOI：
10.1016/j.brainres.2007.12.020
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发表时间：
2008-02-01
期刊：
影响因子：
2.9
通讯作者：
Davis, Fred C.
中科院分区：
文献类型：
--
作者：
Kabrita, Colette S.;Davis, Fred C.
哺乳动物视交叉上核（SCN）是主要的昼夜节律起搏器，负责协调各种生理和行为过程对环境刺激的反应。它由具有独特空间组织的异质细胞群组成，这些空间组织在物种之间可能会有所不同，但通常在两个主要区域的框架内进行讨论，即核的腹外侧或背内侧半部分，或者在其他情况下，核和壳。在仓鼠和大鼠中，不同SCN区域的细胞已被证明具有不同的发育历史。以溴脱氧尿苷为细胞分裂标志物，研究了小鼠（C57 BL/6）SCN细胞起源的时间及其在细胞核内的沉降模式。结果表明，SCN细胞发生发生在胚胎第12和15天之间，并在出生前5天完成。在胚胎第12天出生的细胞主要局限于中SCN的腹外侧区域，而在胚胎第13.5和14.5天产生的细胞在第一个产生的细胞周围形成帽，并延伸到核的后端和前端。这些结果表明，一个有序的SCN细胞发生的时空程序，其中一个中期SCN核心出生第一，其次是周围的壳后出生的细胞。细胞发生的变化可能会影响不同SCN区域的相对大小，从而影响其功能。有丝分裂后细胞离开生殖上皮后，细胞发生和细胞间相互作用的高度有序程序的相对贡献仍有待确定。(c)2007 Elsevier B. V.保留所有权利。
The suprachiasmatic nucleus (SCN) in mammals functions as the principal circadian pacemaker synchronizing diverse physiological and behavioral processes to environmental stimuli. It consists of heterogeneous populations of cells with unique spatial organization that can vary among species, but are commonly discussed within a framework of two principal regions, the ventrolateral or dorsomedial halves of the nucleus or in other instances the core and shell. In both hamsters and rats, cells of different SCN regions have been shown to have different developmental histories. Using bromodeoxyuridine as a marker of cell division, the present study investigated the time of SCN cell origin in mice (C57BL/6) and their settling patterns within the nucleus. Results show that SCN cytogenesis occurs between embryonic days 12 and 15 and is complete 5 days prior to birth. Cells born on embryonic day 12 are mainly confined to a ventrolateral region of the mid-SCN, whereas cells produced later on embryonic days 13.5 and 14.5 form a cap around the cells produced first and extend into the posterior and anterior ends of the nucleus. These results suggest an ordered spatiotemporal program of SCN cytogenesis whereby a mid-SCN core is born first followed by a surrounding shell of later-born cells. Variations in cytogenesis could affect the relative sizes of different SCN regions and, thereby, affect its function. The relative contributions of a highly ordered program of cytogenesis and intercellular interactions after postmitotic cells leave the germinal epithelium remain to be determined. (c) 2007 Elsevier B.V. All rights reserved.