ORGANIZATION OF THE GABAERGIC SYSTEM IN THE RAT HIPPOCAMPAL-FORMATION - A QUANTITATIVE IMMUNOCYTOCHEMICAL STUDY
ORGANIZATION OF THE GABAERGIC SYSTEM IN THE RAT HIPPOCAMPAL-FORMATION - A QUANTITATIVE IMMUNOCYTOCHEMICAL STUDY
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DOI：
10.1002/cne.902800207
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发表时间：
1989-02-08
影响因子：
2.5
通讯作者：
BENARI, Y
中科院分区：
文献类型：
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作者：
WOODSON, W;NITECKA, L;BENARI, Y
使用针对γ氨基丁酸（GABA）和谷氨酸脱羧酶（GAD）的特异性抗体来研究大鼠海马结构中GABA能系统的组织。在半薄切片中评估 GABA 样免疫反应 (Li) 胞体的数量和神经纤维密度。细胞计数显示，大约 11% 的海马神经元群在切片的各个平面内显示 GABA-Li。海马结构的每一层都有 GABA-Li 元素的特征组织。在阿蒙角中，顶端和基底树突区域内 80-95% 的神经元胞体呈 GABA-Li 阳性。在锥体细胞层内，阿蒙角的 CA1 至 CA3 亚区中 5-8% 的细胞是 GABA-Li，而在插入门的锥体细胞层部分中，只有 3% 是 GABA-Li。在 CA1-CA3 树突区域内仅观察到 GABA-Li 神经纤维密度的轻微差异。限制在透明层的是GABA-Li标记的致密带。对位于锥体 (CA1-CA3) 和颗粒体细胞周边的免疫反应颗粒的计数显示，CA3 细胞上的末端纽扣多于 CA1 和颗粒神经元体体上的末端纽扣。在齿状筋膜中发现了 GABA-Li 体细胞和神经纤维的地形分布：标签特别关注其锥体上和头外侧部分。分子层中约 40% 的神经元、多晶型层中约 60% 的神经元以及肺门区域内约 18% 的神经元是 GABA-Li。在颗粒细胞层内，只有 2% 的神经元呈 GABA-Li 阳性。 GABA-Li 神经纤维密度的明显差异存在于齿状筋膜的分子区、细胞周颗粒区和肺门区。虽然 GABA-Li 神经元体细胞的形态根据海马层的不同而不同，但在海马的下区域发现了最异质的细胞类型。在那里，我们发现了一些神经元，这些神经元让人想起 Amaral 和 Woodward 在高尔基体材料中描述的下部区域中间神经元（Brain Res. 124: 225-236, ''77）。此外，特别是在矢状面，我们已经识别出椭圆形、三角形和圆形细胞，并且具有平行排列的突起，似乎沿着颗粒细胞苔藓纤维排列。
Specific antibodies against gamma aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD) were used to study the organization of the GABAergic system in the rat hippocampal formation. Both the number of GABA-like-immunoreactive (Li) somata and neuropil density were assessed in semithin sections. Cell counts revealed that approximately 11% of the hippocampal neuronal population showed GABA-Li within the various planes of section. Each layer in the hippocampal formation had a characteristic organization of GABA-Li elements. In Ammon''s horn, 80-95% of the neuronal somata within the apical and basal dendritic regions were GABA-Li positive. Within the pyramidal cell layer 5-8% of the cells were GABA-Li in the CA1 to CA3 subfields of Ammon''s horn and only 3% were GABA-Li wihtin that portion of the pyramidal cell layer that inserts into the hilus. Only slight differences in the density of the GABA-Li neuropil were observed within the CA1-CA3 dendritic regions. Restricted to the stratum lucidum was a dense band of GABA-Li label. Counts of immunoreactive grains localized on the perimeter of pyramidal (CA1-CA3) and granule somata revealed more terminal boutons on the CA3 cells than on CA1 and granule neuronal somata. A topographical distribution of GABA-Li somata and neuropil was found in the fascia dentata: There the label particularly concerned its suprapyramidal and rostrolateral portions. Approximately 40% of neurons in the molecular layer, 60% in the polymorph layer, and 18% within the hilar region were GABA-Li. Within the granule cell layer only 2% of the neurons were GABA-Li positive. Distinct differences in the density of the GABA-Li neuropil were present in the molecular, pericellular granular, and hilar regions of the fascia dentata. While the morphology of GABA-Li neuronal somata varied according to their hippocampal layer, the most heterogeneous cell types were found in the regio inferior of the hippocampus. There we have identified neurons that are reminiscent of the inferior region interneuron described in Golgi material by Amaral and Woodward (Brain Res. 124: 225-236, ''77). Moreover, particularly in the sagittal plane, we have identified oval, triangular, and round cells and that have processes oriented in a parallel arrangement, appearing to be aligned along the granule cell mossy fibers.