DNA damage and repair following In vitro exposure to two different forms of titanium dioxide nanoparticles on trout erythrocyte
DNA damage and repair following In vitro exposure to two different forms of titanium dioxide nanoparticles on trout erythrocyte
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DOI：
10.1002/tox.20778
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发表时间：
2014-01
影响因子：
4.5
通讯作者：
D. Sekar;Maria Letizia Falcioni;G. Barucca;G. Falcioni
中科院分区：
文献类型：
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作者：
D. Sekar;Maria Letizia Falcioni;G. Barucca;G. Falcioni
TiO2 已被广泛用于促进废弃水溶液中有机化合物的降解，然而，有关 TiO2 纳米颗粒对水生生物毒性的数据仍然有限。在这项体外研究中，我们比较了两种不同家族的 TiO2 纳米颗粒对虹鳟红细胞的毒性。通过 XRD 对两种 TiO2 纳米颗粒的晶体结构进行了分析，结果表明，一种样品由锐钛矿晶相的 TiO2 组成，而另一种样品则包含锐钛矿型和金红石型 TiO2 以 2:8 的比例混合而成的混合物。通过 SEM 高分辨率图像和 BET 技术确定了两个 TiO2 纳米颗粒家族的进一步表征。毒性结果表明，两种 TiO2 纳米粒子均以剂量依赖性方式增加溶血率（1.6、3.2、4.8 μg mL−1），但由于化学发光测量的 NADH 添加，它们不会影响超氧阴离子的产生。此外，TiO2 纳米粒子 (4.8 μg mL−1) 会引起 DNA 损伤，并且损伤的实体与所使用的 TiO2 纳米粒子的类型无关。改进的彗星测定（Endo III 和 Fpg）表明，TiO2 不仅氧化嘌呤，而且氧化嘧啶碱基。在我们的实验条件下，暴露于 TiO2 纳米颗粒不会影响 DNA 修复系统的功能。获得的数据有助于更好地表征与 TiO2 纳米粒子暴露相关的水环境风险。 © 2011 Wiley periodicals, Inc. Environ Toxicol 29: 117–127, 2014。
TiO2 has been widely used to promote organic compounds degradation on waste aqueous solution, however, data on TiO2 nanotoxicity to aquatic life are still limited. In this in vitro study, we compare the toxicity of two different families of TiO2 nanoparticles on erythrocytes from Oncorhynchus mykiss trout. The crystal structure of the two TiO2 nanoparticles was analyzed by XRD and the results indicated that one sample is composed of TiO2 in the anatase crystal phase, while the other sample contains a mixture of both the anatase and the rutile forms of TiO2 in a 2:8 ratio. Further characterization of the two families of TiO2 nanoparticles was determined by SEM high resolution images and BET technique. The toxicity results indicate that both TiO2 nanoparticles increase the hemolysis rate in a dose dependent way (1.6, 3.2, 4.8 μg mL−1) but they do not influence superoxide anion production due to NADH addition measured by chemiluminescence. Moreover, TiO2 nanoparticles (4.8 μg mL−1) induce DNA damage and the entity of the damage is independent from the type of TiO2 nanoparticles used. Modified comet assay (Endo III and Fpg) shows that TiO2 oxidizes not only purine but also pyrimidine bases. In our experimental conditions, the exposure to TiO2 nanoparticles does not affect the DNA repair system functionality. The data obtained contribute to better characterize the aqueous environmental risks linked to TiO2 nanoparticles exposure. © 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 117–127, 2014.