The testosterone metabolite 3α-diol enhances female rat sexual motivation when infused in the nucleus accumbens shell.
The testosterone metabolite 3α-diol enhances female rat sexual motivation when infused in the nucleus accumbens shell.
复制标题
当注入伏核壳时，睾酮代谢物 3α-二醇可增强雌性大鼠的性动机。
DOI：
10.1111/j.1743-6109.2010.01937.x
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Jorge,JuanCarlos
中科院分区：
文献类型：
--
作者：
SánchezMontoya,ElianaL;Hernández,Lizaida;Barreto-Estrada,JenniferL;Ortiz,JoséG;Jorge,JuanCarlos
AimThe purpose of this study was to provide a quantitative assessment of female rat sexual behaviors after acute exposure to the A-ring reduced testosterone metabolite, androstanediol (3α-Diol), through the nucleus accumbens (NA) shell.Main Outcome MeasuresQuantitative analyses of female rat sexual behaviors and assessment of protein levels for the enzyme glutamic acid decarboxylase isoform 67 (GAD67) and gephyrin, a protein that participates in the clustering of GABA-A receptors in postsynaptic cells, were accomplished.MethodsFemale rats were ovariectomized and primed with estrogen and progesterone to induce sexual behaviors. Females received a 3α-Diol infusion via guided cannula that aimed to the NA shell five minutes prior to a sexual encounter with a stud male. The following parameters were videotaped and measured in a frame by frame analysis: lordosis quotient (LQ), Lordosis rating (LR), frequency and duration of proceptive behaviors (hopping/darting and ear wiggling). Levels of GAD67 and gephyrin were obtained by Western blot analysis two or twenty-four hours after the sexual encounter.ResultsAcute exposure to 3α-Diol in the NA shell enhanced LR, ear wiggling, and hopping/darting but not LQ. Some of these behavioral effects were counteracted by co-infusion of 3α-Diol plus the GABAA-receptor antagonist GABAzine. A transient reduction of GAD67 levels in the NA shell was detected.ConclusionsThe testosterone metabolite 3α-Diol enhances sexual proceptivity, but not receptivity, when infused into the NA shell directly. The GABAergic system may participate in the androgen-mediated enhancement of female rat sexual motivation.
