Electrical detection and characterization of bacterial adhesion using electrochemical impedance spectroscopy-based flow chamber
Electrical detection and characterization of bacterial adhesion using electrochemical impedance spectroscopy-based flow chamber
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DOI：
10.1016/j.colsurfa.2008.01.005
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发表时间：
2008-04-01
影响因子：
5.2
通讯作者：
Ben Ouada, Hafedh
中科院分区：
文献类型：
--
作者：
Bayoudh, Sonia;Othmane, Ali;Ben Ouada, Hafedh
在目前的工作中，我们报告的电化学检测和表征细菌粘附到半导体氧化铟锡（ITO）板使用电化学阻抗谱（EIS）为基础的流动室。我们使用两种不同的细菌菌株（施氏假单胞菌（PS）和表皮葡萄球菌（SE）），以便它们的粘附行为和电荷传输性能进行比较。通过监测两种细菌菌株粘附过程中低频范围内的阻抗变化来实现电检测。通过在粘附2小时之前和之后测量大频率范围内的阻抗来实现电表征。的电极/细菌/电解质接口的电性能进行了解释的等效电路的电阻和电容，其频率依赖的阻抗拟合到测得的数据曲线。阻抗的大小被发现呈指数衰减的粘附细胞的数量增加，在沉积时间为两种细菌细胞。PS菌的粘附先于SE菌的粘附。此外，阻抗拟合结果显示，PS细菌细胞比SE细菌细胞允许更多的电荷转移到电极，因此它提供更多的电荷，并更快，更牢固地粘附到ITO表面。(C)2008 Elsevier B. V.保留所有权利。
In the present work, we report on the electrochemical detection and characterization of bacterial adhesion onto a semiconducting indium tin oxide (ITO) plate using an electrochemical impedance spectroscopy (EIS)-based flow chamber. We used two different bacterial strains (Pseudomonas stutzeri (PS) and Staphylococcus epidermidis (SE)) so that their adhesion behavior and charge transporting property could be compared. The electrical detection was achieved by monitoring the impedance variations in the low frequency range during the adhesion process of both bacterial strains. The electrical characterization was achieved by measuring the impedance over a large frequency range before and after 2 hr of adhesion. The electrical properties of the electrode/bacteria/electrolyte interfaces were explained in terms of resistances and capacitances of an equivalent circuit whose frequency-dependant impedance was fitted to the measured data curves. The magnitude of the impedance was found to decay exponentially as the number of adhering cells increased during the deposition time for both bacterial cells. The adhesion of PS bacteria was detected electrically before SE bacteria. Also, the impedance fitting results revealed that PS bacterial cell allow more charge transfer to the electrode than SE bacterial cell, and therefore it donates more charges and adheres faster and more firmly to ITO surface. (C) 2008 Elsevier B.V. All rights reserved.