Unique expression pattern of KIBRA in the enteric nervous system of APP/ PS1 mice
Unique expression pattern of KIBRA in the enteric nervous system of APP/ PS1 mice
复制标题
KIBRA 在 APP/PS1 小鼠肠神经系统中的独特表达模式。
DOI：
10.1016/j.neulet.2018.03.014
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发表时间：
2018-05-14
影响因子：
2.5
通讯作者：
Du, Yifeng
中科院分区：
文献类型：
--
作者：
Feng, Jianli;Dong, Lingling;Du, Yifeng
Kibra被认为是与记忆相关的基因，在哺乳动物的大脑和肾脏中含量丰富。然而Kibra在“第二脑”--肠道神经系统(ENS)中的表达模式尚不清楚，尤其是在神经退行性疾病中。本研究旨在通过整体染色技术研究Kibra在APP/PS1小鼠和野生型小鼠肠道肌间神经丛中的详细表达模式。APP/PS1小鼠肠肌间神经丛中AP沉积，磷酸化Tau(p-Tau)和总Tau(T-Tau)蛋白水平升高。有趣的是，Tuj-1细胞的数量在这两组中保持不变。与对照组相比，APP/PS1小鼠空肠肌间神经丛Kibra蛋白表达显著增加，空肠肌间神经丛和皮质Kibra+GABA能神经元比例明显增加。APP/PS1小鼠与野生型小鼠相比，Kibra+胆碱能神经元和Kibra+氮能神经元数量无明显差异。综上所述，我们的研究进一步证实了阿尔茨海默病(AD)的典型病理特征不仅存在于中枢神经系统，而且存在于ENS中。
KIBRA has been recognized as a memory-related gene, which is abundant in the brain and kidney of mammals. However, the expression pattern of KIBRA in the "second brain"-enteric nervous system (ENS) is still unknown, especially in neurodegenerative disorders. In this study, we aimed to investigate the detailed expression pattern of KIBRA in the intestinal myenteric nerve plexus of APP/PS1 and wild type mice by whole mount staining technology. The deposition of AP and increased levels of phosphorylated Tau (p-Tau) and total Tau (T-Tau) protein were observed in the intestinal myenteric nerve plexus of APP/PS1 mice. Interestingly, the amount of Tuj -1- cells remained unchanged between these two groups. Compared to the control mice, the protein levels of KIBRA significantly increased in the jejunal myenteric plexus of APP/PS1 mice, and the proportion of KIBRA + GABAergic neurons in both the jejunal myenteric nerve plexus and the cortex was much higher in the APP/PS1 mice. But there was no significant difference in the number of KIBRA + cholinergic neurons and KIBRA + nitrergic neurons between APP/PS1 and wild type mice. In summary, our study further confirmed that typical pathology features of Alzheimer's disease (AD) not only existed in the central nervous system but also in the ENS.