Styrene-Maleic Acid Copolymer Effects on the Function of the GPCR Rhodopsin in Lipid Nanoparticles.
Styrene-Maleic Acid Copolymer Effects on the Function of the GPCR Rhodopsin in Lipid Nanoparticles.
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DOI：
10.1016/j.bpj.2021.09.012
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发表时间：
2021-09
影响因子：
3.4
通讯作者：
I. Szundi;Stephanie G. Pitch;E. Chen;D. Farrens;D. Kliger
中科院分区：
文献类型：
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作者：
I. Szundi;Stephanie G. Pitch;E. Chen;D. Farrens;D. Kliger
苯乙烯-马来酸（SMA）共聚物可溶解生物膜，形成脂质纳米颗粒（SMALPs），其中含有被天然脂质包围的膜蛋白，因此可以使用各种生物物理技术进行结构和功能研究。然而，smalp是否提供了一个真正的自然环境或SMA增溶作用是否影响膜蛋白的功能特性的问题仍然是开放的。我们通过比较视紫红质的光活化动力学来解决这个问题，视紫红质是一种g蛋白偶联受体，存在于杆状细胞的盘膜上，在天然膜和SMALPs中以不同的SMA（3:1）和视紫红质的摩尔比制备。时间分辨吸收光谱结合复杂动力学分析揭示了天然膜和sma稳定环境之间的动力学和机理差异。结果表明纳米粒子的摩尔比范围适合动力学研究。
Styrene-maleic acid (SMA) copolymers solubilize biological membranes to form lipid nanoparticles (SMALPs) that contain membrane proteins surrounded by native lipids, thus enabling the use of a variety of biophysical techniques for structural and functional studies. The question of whether SMALPs provide a truly natural environment or SMA solubilization affects the functional properties of membrane proteins, however, remains open. We address this question by comparing the photoactivation kinetics of rhodopsin, a G-protein-coupled receptor in the disk membranes of rod cells, in native membrane and SMALPs prepared at different molar ratios between SMA(3:1) and rhodopsin. Time-resolved absorption spectroscopy combined with complex kinetic analysis reveals kinetic and mechanistic differences between the native membrane and SMA-stabilized environment. The results suggest a range of molar ratios for nanoparticles suitable for kinetic studies.