The number of neurons in Drosophila and mosquito brains.
The number of neurons in Drosophila and mosquito brains.
复制标题
DOI：
10.1371/journal.pone.0250381
复制
发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Potter CJ
中科院分区：
文献类型：
--
作者：
Raji JI;Potter CJ
各种昆虫物种作为研究大脑控制复杂行为的细胞和分子机制的有价值的模型系统。特别是，果蝇的神经系统已经得到了广泛的研究，但旨在确定果蝇大脑中神经元数量的实验却令人惊讶地缺乏。应用各向同性分离器结合免疫组织化学技术，对果蝇全脑、中枢和视叶的神经元和非神经元细胞总数进行了计数。为了比较，我们还计算了三种不同的蚊子：埃及伊蚊，按蚊coluzzii和致倦库蚊的神经元种群。成年人全脑神经元的平均数量为199，380 ± 3，400个。黑腹型217，910 ± 6，180个细胞;埃及伊蚊223，020 ± 4，650个细胞。coluzzii和225，911 ± 7，220个细胞。致倦肌D.中枢与视叶的平均神经元细胞计数。黑腹型（101，140 ± 3，650 vs.107，270 ± 2，720），Ae.埃及伊蚊（109，140 ± 3，550 vs.112，000 ± 4，280），埃及按蚊（109，140 ± 3，550 vs.112，000 ± 4，280）; coluzzii（105，130 ± 3，670 vs.107，140 ± 3，090）和C.还估计了致倦犬（108，530 ± 7，990 vs. 110，670 ± 3，950）。每个昆虫脑由其总细胞群的89% ± 2%的神经元组成。各向同性分馏分析没有发现明显的性二型性的神经元和非神经元细胞群体的这些昆虫。我们的研究为果蝇和蚊子脑中神经元的总数提供了实验证据。
Various insect species serve as valuable model systems for investigating the cellular and molecular mechanisms by which a brain controls sophisticated behaviors. In particular, the nervous system of Drosophila melanogaster has been extensively studied, yet experiments aimed at determining the number of neurons in the Drosophila brain are surprisingly lacking. Using isotropic fractionator coupled with immunohistochemistry, we counted the total number of neuronal and non-neuronal cells in the whole brain, central brain, and optic lobe of Drosophila melanogaster. For comparison, we also counted neuronal populations in three divergent mosquito species: Aedes aegypti, Anopheles coluzzii and Culex quinquefasciatus. The average number of neurons in a whole adult brain was determined to be 199,380 ±3,400 cells in D. melanogaster, 217,910 ±6,180 cells in Ae. aegypti, 223,020 ± 4,650 cells in An. coluzzii and 225,911±7,220 cells in C. quinquefasciatus. The mean neuronal cell count in the central brain vs. optic lobes for D. melanogaster (101,140 ±3,650 vs. 107,270 ± 2,720), Ae. aegypti (109,140 ± 3,550 vs. 112,000 ± 4,280), An. coluzzii (105,130 ± 3,670 vs. 107,140 ± 3,090), and C. quinquefasciatus (108,530 ±7,990 vs. 110,670 ± 3,950) was also estimated. Each insect brain was comprised of 89% ± 2% neurons out of its total cell population. Isotropic fractionation analyses did not identify obvious sexual dimorphism in the neuronal and non-neuronal cell population of these insects. Our study provides experimental evidence for the total number of neurons in Drosophila and mosquito brains.
登录
查看更多内容
影响因子：
2.9
作者：
Napper RMA
通讯作者：
Napper RMA
影响因子：
7.7
作者：
Basrur NS;De Obaldia ME;Morita T;Herre M;von Heynitz RK;Tsitohay YN;Vosshall LB
通讯作者：
Vosshall LB
影响因子：
3.3
作者：
McGurk, Leeanne;Berson, Amit;Bonini, Nancy M.
通讯作者：
Bonini, Nancy M.
影响因子：
2.5
作者：
Azevedo, Frederico A. C.;Carvalho, Ludmila R. B.;Herculano-Houzel, Suzana
通讯作者：
Herculano-Houzel, Suzana
影响因子：
3
作者：
Neves, Kleber;Guimaraes, Daniel Menezes;Mota, Bruno
通讯作者：
Mota, Bruno