Surface characterizations of mono-, di-, and tri-aminosilane treated glass substrates
Surface characterizations of mono-, di-, and tri-aminosilane treated glass substrates
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DOI：
10.1016/j.jcis.2006.03.045
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发表时间：
2006-06-15
影响因子：
9.9
通讯作者：
Pantano, C. G.
中科院分区：
文献类型：
--
作者：
Metwalli, E.;Haines, D.;Pantano, C. G.
采用X射线光电子能谱（XPS）、拉曼光谱、原子力显微镜（AFM）和流动电位等表面分析技术研究了单、二和三氨基硅烷处理的玻璃表面的表面性质和结构。一个优化的浸涂工艺被证明产生大致硅烷单层覆盖在玻璃表面上。表面电荷的测量表明，ammosilanization类表面从负电位转换为正电位在中性pH值。较高的正流电位，观察到与单和二氨基硅烷处理的玻璃表面相比，三。适用于所有氨基硅烷处理的玻璃样品。高分辨率N1sXPS光谱表明质子化的氨基优先朝向玻璃表面取向，而游离氨基向外突出。本研究的目的是获得均匀的，可重复的薄，强粘附性，内部交联，高正电荷的氨基硅烷涂层的玻璃表面用于连接的DNA片段用于微阵列实验。(c)2006年爱思唯尔公司All rights reserved.
The surface properties and structure of mono-, di-, and tri-aminosilane treated glass surfaces were investigated using surface analytical techniques including X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, atomic force microscopy (AFM), and streaming potential. An optimized dip-coating process was demonstrated to produce roughly silane monolayer coverage on the glass surface. The surface charge measurements indicated that ammosilanization converts the class surface from negative to positive potentials at neutral pH values. Higher positive streaming potential was observed for tri- compared with mono- and di-aminosilane treated glass surfaces. For all aminosilane treated glass samples. the high-resolution N 1s XPS spectra indicated a preferential orientation of the protonated amino-groups towards the glass surface whereas the free amino groups were protruding outward. This study aimed to obtain uniform, reproducibly thin, strongly adhering, internally cross-linked, and high positively charged aminosilane-coated glass surfaces for the attachment of DNA fragments used in microarraying experiments. (c) 2006 Elsevier Inc. All rights reserved.